https://scholars.lib.ntu.edu.tw/handle/123456789/578157
標題: | A proteomic approach identifies early pregnancy biomarkers for preeclampsia: Novel linkages between a predisposition to preeclampsia and cardiovascular disease | 作者: | Blumenstein, M McMaster, M.T Black, M.A Wu, S Prakash, R Cooney, J McCowan, L.M.E Cooper, G.J.S North, R.A. STEVEN HUNG-HSI WU |
關鍵字: | DIGE | Plasma biomarkers | Preeclampsia | Pregnancy | Small for gestational age | 公開日期: | 2009 | 出版社: | Wiley | 卷: | 9 | 期: | 11 | 來源出版物: | PROTEOMICS | 摘要: | Preeclampsia (PE) is a common, potentially life-threatening pregnancy syndrome triggered by placental factors released into the maternal circulation, resulting in maternal vascular dysfunction along with activated inflammation and coagulation. Currently there is no screening test for PE. We sought to identify differentially expressed plasma proteins in women who subsequently develop PE that may perform as predictive biomarkers. In seven DIGE experiments, we compared the plasma proteome at 20wk gestation in women who later developed PE with an appropriate birth weight for gestational age baby (n=27) or a small for gestational age baby (n=12) to healthy controls with uncomplicated pregnancies (n=57). Of the 49 differentially expressed spots associated with PE-appropriate for gestational age, PE-small for gestational age or both (p<0.05, false discovery rate corrected), 39 were identified by LC-MS/MS. Two protein clusters that accurately (>90%) classified women at risk of developing PE were identified. Immunoblots confirmed the overexpression of fibrinogen γ chain and α-1-antichymotrypsin in plasma prior to PE. The proteins identified are involved in lipid metabolism, coagulation, complement regulation, extracellular matrix remodeling, protease inhibitor activity and acute-phase responses, indicating novel synergism between pathways involved in the pathogenesis of PE. Our findings are remarkably similar to recently identified proteins complexed to high-density lipoprotein and linked to cardiovascular disease. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/578157 | ISSN: | 1615-9853 | DOI: | 10.1002/pmic.200800625 |
顯示於: | 農藝學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。