https://scholars.lib.ntu.edu.tw/handle/123456789/578847
標題: | Bacterial diversity among four healthcare-associated institutes in Taiwan | 作者: | Chen C.-H. Lin Y.-L. Chen K.-H. Chen W.-P. Chen Z.-F. HAN-YUEH KUO Hung H.-F. Tang C.Y. Liou M.-L. |
公開日期: | 2017 | 出版社: | Nature Publishing Group | 卷: | 7 | 期: | 1 | 起(迄)頁: | 8230 | 來源出版物: | Scientific Reports | 摘要: | Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs. ? 2017 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027581123&doi=10.1038%2fs41598-017-08679-3&partnerID=40&md5=b55c791b777514cb5214fcbd488bf7d6 https://scholars.lib.ntu.edu.tw/handle/123456789/578847 |
ISSN: | 2045-2322 | DOI: | 10.1038/s41598-017-08679-3 | SDG/關鍵字: | RNA 16S; bacterium; biodiversity; classification; clinical trial; cross infection; environmental microbiology; genetics; health care facility; high throughput sequencing; human; metagenomics; microbiology; microflora; multicenter study; phylogeny; procedures; Taiwan; Bacteria; Biodiversity; Cross Infection; Environmental Microbiology; Health Facilities; High-Throughput Nucleotide Sequencing; Humans; Metagenomics; Microbiota; Phylogeny; RNA, Ribosomal, 16S; Taiwan |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。