https://scholars.lib.ntu.edu.tw/handle/123456789/579853
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wu, Chih Yu | en_US |
dc.contributor.author | YU-CHIH CHIANG | en_US |
dc.contributor.author | Christy, Jane | en_US |
dc.contributor.author | ABEL PO-HAO HUANG | en_US |
dc.contributor.author | Chang, Nai Yun | en_US |
dc.contributor.author | Wenny, Chiu Y.-C. | en_US |
dc.contributor.author | Chiu, Yu Chih | en_US |
dc.contributor.author | Yang, Yen Ching | en_US |
dc.contributor.author | Chen, Po Chun | en_US |
dc.contributor.author | Wang, Peng Yuan | en_US |
dc.contributor.author | HSIEN-YEH CHEN | en_US |
dc.date.accessioned | 2021-08-30T02:37:13Z | - |
dc.date.available | 2021-08-30T02:37:13Z | - |
dc.date.issued | 2021-05-01 | - |
dc.identifier.issn | 2079-6412 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/579853 | - |
dc.description.abstract | Modifications of biomaterials based on the combination of physical, chemical, and bio-logical cues for manipulating stem cell growth are needed for modern regenerative medicine. The exploitation of these sophisticated modifications remains a challenge, including substrate limitation, biocompatibility, and versatile and general cues for stem cell activities. In this report, a vapor-phase coating technique based on the functionalization of poly-p-xylylene (PPX) was used to generate a surface modification for use with stem cells in culture. The coating provided the ability for covalent conjugation that immobilized bone morphogenetic protein 2 (BMP-2) and fibroblast growth factor 2 (FGF-2), and the modified coating surfaces enabled direct stem cell differentiation and controlled proliferation because of the specific activities. The ligations were realized between the growth factors and the maleimide-modified surface, and the conjugation reactions proceeded with high specificity and rapid kinetics under mild conditions. The conjugation densities were approximately 140 ng·cm−2 for BMP-2 and 155 ng·cm−2 for FGF-2. Guiding the activities of the human adipose-derived stem cells (hADSCs) was achieved by modifying surfaces to promote the hADSC differentiation capacity and proliferation rate. The reported coating system demonstrated biocompatibility, substrate-independent conformity, and stability, and it could provide an effective and versatile interface platform for further use in biomedical applications. | en_US |
dc.relation.ispartof | Coatings | en_US |
dc.subject | Biointerface | CVD polymerization | Growth factor | Stem cells | Surface modification | en_US |
dc.subject | Biointerface; CVD polymerization; Growth factor; Stem cells; Surface modification | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Guiding stem cell differentiation and proliferation activities based on nanometer-thick functionalized poly-p-xylylene coatings | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/coatings11050582 | - |
dc.identifier.scopus | 2-s2.0-85106999094 | - |
dc.identifier.url | https://scholars.lib.ntu.edu.tw/handle/123456789/570004 | - |
dc.relation.journalvolume | 11 | en_US |
dc.relation.journalissue | 5 | en_US |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Dentistry | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Chemical Engineering | - |
crisitem.author.orcid | 0000-0001-8110-3033 | - |
crisitem.author.orcid | 0000-0002-5689-6761 | - |
crisitem.author.orcid | 0000-0001-5956-6680 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 醫學系 |
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