https://scholars.lib.ntu.edu.tw/handle/123456789/582869
標題: | Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation | 作者: | Lin, Hung Wei Lee, Jin Yu Chou, Nai Lin Shih, Ting Wei MAU-SUN CHANG |
公開日期: | 1-八月-2021 | 出版社: | Public Library of Science | 卷: | 16 | 期: | 8 August | 起(迄)頁: | Article number e0256282 | 來源出版物: | PLoS ONE | 摘要: | Human PUF-A/PUM3 is a RNA and DNA binding protein participating in the nucleolar processing of 7S to 5.8S rRNA. The nucleolar localization of PUF-A redistributes to the nucleoplasm upon the exposure to genotoxic agents in cells. However, little is known regarding the roles of PUF-A in tumor progression. Phosphoprotein database analysis revealed that Y259 phosphorylation of PUF-A is the most prevalent residue modified. Here, we reported the importance of PUF-A's phosphorylation on Y259 in tumorigenesis. PUF-A gene was knocked out by the Crispr/Cas9 method in human cervix epithelial HeLa cells. Loss of PUF-A in HeLa cells resulted in reduced clonogenic and lower transwell invasion capacity. Introduction of PUF-AY259F to PUF-A deficient HeLa cells was unable to restore colony formation. In addition, the unphosphorylated mutant of PUF-A, PUF-AY259F, attenuated PUF-A protein stability. Our results suggest the important role of Y259 phosphorylation of PUF-A in cell proliferation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/582869 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0256282 | SDG/關鍵字: | minor histocompatibility antigen; phosphoprotein; PUM3 protein, human; tyrosine; book; carcinogenesis; cell motion; cell proliferation; CRISPR Cas system; female; gene deletion; genetic database; genetics; HeLa cell line; human; metabolism; mortality; mutation; neoplasm; nucleolus; pathology; phosphorylation; protein processing; protein stability; survival analysis; Atlases as Topic; Carcinogenesis; Cell Movement; Cell Nucleolus; Cell Proliferation; CRISPR-Cas Systems; Databases, Genetic; Female; Gene Deletion; HeLa Cells; Humans; Minor Histocompatibility Antigens; Mutation; Neoplasms; Phosphoproteins; Phosphorylation; Protein Processing, Post-Translational; Protein Stability; Survival Analysis; Tyrosine |
顯示於: | 生化科學研究所 |
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