https://scholars.lib.ntu.edu.tw/handle/123456789/582936
標題: | Quantitative phosphoproteomics reveals cell alignment and mitochondrial length change under cyclic stretching in lung cells | 作者: | Wang, W.-H. CHIA-LANG HSU Huang, H.-C. Juan, H.-F. |
公開日期: | 2020 | 出版社: | MDPI AG | 卷: | 21 | 期: | 11 | 起(迄)頁: | 1-17 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Lung cancer is a leading cause of death. Most previous studies have been based on traditional cell-culturing methods. However, lung cells are periodically subjected to mechanical forces during breathing. Understanding the mechanisms underlying the cyclic stretching induced in lung cells may be important for lung cancer therapy. Here, we applied cyclic stretching to stimulate the continual contraction that is present under physiological conditions in lung cells. We first uncovered the stretching-induced phosphoproteome in lung cancer cell line A549 and fibroblast cell line IMR-90. We identified 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins in A549 and IMR-90, respectively. Furthermore, we combined our phosphoproteomics and public gene expression data to identify the biological functions in response to cyclic stretching. Interestingly, cytoskeletal and mitochondrial reorganization were enriched. We further used cell imaging analysis to validate the profiling results and found that this physical force changed cell alignment and mitochondrial length. This study not only reveals the molecular mechanism of cyclic stretching but also provides evidence that cell stretching causes cellular rearrangement and mitochondrial length change. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086154825&doi=10.3390%2fijms21114074&partnerID=40&md5=6348504ba7859a9cfb4f47fc8e2be678 https://scholars.lib.ntu.edu.tw/handle/123456789/582936 |
ISSN: | 1661-6596 | DOI: | 10.3390/ijms21114074 | SDG/關鍵字: | phosphopeptide; proteome; phosphoprotein; proteome; A-549 cell line; Article; cell survival; data analysis; enzyme specificity; extraction; gene expression; human; human cell; immunocytochemistry; immunofluorescence test; immunohistochemistry; IMR-90 cell line; information processing; liquid chromatography-mass spectrometry; live cell imaging; lung alveolus cell; phosphoproteomics; protein analysis; protein phosphorylation; protein processing; stretching; biological model; cell culture; cell line; cytology; cytoskeleton; gene expression regulation; lung; mechanical stress; metabolism; mitochondrion; phosphorylation; procedures; proteomics; ultrastructure; Cell Line; Cells, Cultured; Cytoskeleton; Gene Expression Regulation; Humans; Lung; Mitochondria; Models, Biological; Phosphoproteins; Phosphorylation; Proteome; Proteomics; Stress, Mechanical |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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