https://scholars.lib.ntu.edu.tw/handle/123456789/582947
標題: | RNA-Binding Proteomics Reveals MATR3 Interacting with lncRNA SNHG1 to Enhance Neuroblastoma Progression | 作者: | Yang T.-W. Sahu D. Chang Y.-W. CHIA-LANG HSU Hsieh C.-H. Huang H.-C. Juan H.-F. |
公開日期: | 2019 | 出版社: | American Chemical Society | 卷: | 18 | 期: | 1 | 起(迄)頁: | 406-416 | 來源出版物: | Journal of Proteome Research | 摘要: | The interaction of long noncoding RNAs (lncRNAs) with one or more RNA-binding proteins (RBPs) is important to a plethora of cellular and physiological processes. The lncRNA SNHG1 was reported to be aberrantly expressed and associated with poor patient prognosis in several cancers including neuroblastoma. However, the interacting RBPs and biological functions associated with SNHG1 in neuroblastoma remain unknown. In this study, we identified 283, 31, and 164 SNHG1-interacting proteins in SK-N-BE(2)C, SK-N-DZ, and SK-N-AS neuroblastoma cells, respectively, using a RNA-protein pull-down assay coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Twenty-four SNHG1-interacting RBPs were identified in common from these three neuroblastoma cell lines. RBPs MATR3, YBX1, and HNRNPL have the binding sites for SNHG1 predicted by DeepBind motif analysis. Furthermore, the direct binding of MATR3 with SNHG1 was validated by Western blot and confirmed by RNA immunoprecipitation assay (RIP). Coexpression analysis revealed that the expression of SNHG1 is positively correlated with MATR3 (P = 3.402 × 10 -13 ). The high expression of MATR3 is associated with poor event-free survival (P = 0.00711) and overall survival (P = 0.00064). Biological functions such as ribonucleoprotein complex biogenesis, RNA processing, and RNA splicing are significantly enriched and in common between SNHG1 and MATR3. In conclusion, we identified MATR3 as binding to SNHG1 and the interaction might be involved in splicing events that enhance neuroblastoma progression. ? Copyright 2018 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058872934&doi=10.1021%2facs.jproteome.8b00693&partnerID=40&md5=091b252defe91f2d51f5de460e967948 https://scholars.lib.ntu.edu.tw/handle/123456789/582947 |
ISSN: | 1535-3893 | DOI: | 10.1021/acs.jproteome.8b00693 | SDG/關鍵字: | 60S ribosomal protein L19; 60S ribosomal protein L22; 60S ribosomal protein L3; 60S ribosomal protein L6; aly ref export factor 2; guanosine triphosphatase activating protein; heterogeneous nuclear ribonucleoprotein; heterogeneous nuclear ribonucleoprotein A0; heterogeneous nuclear ribonucleoprotein AB; heterogeneous nuclear ribonucleoprotein D; heterogeneous nuclear ribonucleoprotein group M; heterogeneous nuclear ribonucleoprotein L; heterogeneous nuclear ribonucleoprotein Q; histone; histone h1x; interferon; interferon induced protein with tetratricopeptide repeats 5; interleukin enhancer binding factor 3; long untranslated RNA; matrilysin; matrin 3; non pou domain containing octamer binding protein; retinoic acid inducible protein I; ribosome protein; RNA binding protein; small nuclear ribonucleoprotein; small nuclear ribonucleoprotein sm d1; small nuclear ribonucleoprotein sm d3; small nucleolar RNA host gene 1; unclassified drug; Y box binding protein 1; long non-coding RNA SNHG1, human; long untranslated RNA; MATR3 protein, human; nuclear matrix protein; protein binding; RNA binding protein; 60S ribosomal subunit; Article; binding site; cancer growth; cancer survival; carcinogenesis; comparative study; controlled study; event free survival; gene expression; human; human cell; liquid chromatography-mass spectrometry; major clinical study; molecular pathology; neuroblastoma; neuropathology; overall survival; priority journal; protein RNA binding; proteomics; RNA degradation; RNA processing; RNA splicing; SK-N-AS cell line; SK-N-BE(2)-C cell line; SK-N-DZ cell line; upregulation; disease exacerbation; metabolism; mortality; neuroblastoma; pathology; procedures; proteomics; survival analysis; tumor cell line; Cell Line, Tumor; Disease Progression; Humans; Neuroblastoma; Nuclear Matrix-Associated Proteins; Protein Binding; Proteomics; RNA Splicing; RNA, Long Noncoding; RNA-Binding Proteins; Survival Analysis |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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