https://scholars.lib.ntu.edu.tw/handle/123456789/582964
標題: | Temporal phosphoproteome dynamics induced by an ATP synthase inhibitor citreoviridin | 作者: | Hu C.-W. CHIA-LANG HSU Wang Y.-C. Ishihama Y. Ku W.-C. Huang H.-C. Juan H.-F. |
公開日期: | 2015 | 出版社: | American Society for Biochemistry and Molecular Biology Inc. | 卷: | 14 | 期: | 12 | 起(迄)頁: | 3284-3298 | 來源出版物: | Molecular and Cellular Proteomics | 摘要: | Citreoviridin, one of toxic mycotoxins derived from fungal species, can suppress lung cancer cell growth by inhibiting the activity of ectopic ATP synthase, but has limited effect on normal cells. However, the mechanism of citreoviridin triggering dynamic molecular responses in cancer cells remains unclear. Here, we performed temporal phosphoproteomics to elucidate the dynamic changes after citreoviridin treatment in cells and xenograft model. We identified a total of 829 phosphoproteins and demonstrated that citreoviridin treatment affects protein folding, cell cycle, and cytoskeleton function. Furthermore, response network constructed by mathematical modeling shows the relationship between the phosphorylated heat shock protein 90 β and mitogen-activated protein kinase signaling pathway. This work describes that citreoviridin suppresses cancer cell growth and mitogenactivated protein kinase/extracellular signal-regulated kinase signaling by site-specific dephosphorylation of HSP90AB1 on Serine 255 and provides perspectives in cancer therapeutic strategies. ? 2015 by The American Society for Biochemistry and Molecular Biology, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84948654284&doi=10.1074%2fmcp.M115.051383&partnerID=40&md5=17ba55dd3138c345f498cfe1484b242e https://scholars.lib.ntu.edu.tw/handle/123456789/582964 |
ISSN: | 1535-9476 | DOI: | 10.1074/mcp.M115.051383 | SDG/關鍵字: | citreoviridin; heat shock protein 90; heat shock protein 90 beta; mitogen activated protein kinase; mycotoxin; phosphoprotein; proton transporting adenosine triphosphate synthase inhibitor; serine; serine 255; unclassified drug; aurovertin; citreoviridin; enzyme inhibitor; heat shock protein 90; HSP90AB1 protein, human; proteome; animal experiment; animal model; animal tissue; Article; cancer growth; cell viability; controlled study; dephosphorylation; human; human cell; mouse; nonhuman; priority journal; protein folding; protein phosphorylation; proteomics; signal transduction; animal; cell cycle; chemistry; drug effects; drug screening; gene expression regulation; genetics; Lung Neoplasms; metabolism; phosphorylation; theoretical model; tumor cell line; Animals; Aurovertins; Cell Cycle; Cell Line, Tumor; Enzyme Inhibitors; Gene Expression Regulation, Neoplastic; HSP90 Heat-Shock Proteins; Humans; Lung Neoplasms; MAP Kinase Signaling System; Mice; Models, Theoretical; Phosphorylation; Protein Folding; Proteome; Xenograft Model Antitumor Assays |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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