https://scholars.lib.ntu.edu.tw/handle/123456789/582969
標題: | Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells | 作者: | Chang H.-Y. Li M.-H. Huang T.-C. CHIA-LANG HSU Tsai S.-R. Lee S.-C. Huang H.-C. Juan H.-F. |
公開日期: | 2015 | 出版社: | American Chemical Society | 卷: | 14 | 期: | 2 | 起(迄)頁: | 1250-1262 | 來源出版物: | Journal of Proteome Research | 摘要: | Breast cancer is one of the leading cancer-related causes of death worldwide. Treatment of triple-negative breast cancer (TNBC) is complex and challenging, especially when metastasis has developed. In this study, we applied infrared radiation as an alternative approach for the treatment of TNBC. We used middle infrared (MIR) with a wavelength range of 3-5 ?m to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells but did not affect the growth of normal breast epithelial cells. We performed iTRAQ-coupled LC-MS/MS analysis to investigate the MIR-triggered molecular mechanisms in breast cancer cells. A total of 1749 proteins were identified, quantified, and subjected to functional enrichment analysis. From the constructed functionally enriched network, we confirmed that MIR caused G2/M cell cycle arrest, remodeled the microtubule network to an astral pole arrangement, altered the actin filament formation and focal adhesion molecule localization, and reduced cell migration activity and invasion ability. Our results reveal the coordinative effects of MIR-regulated physiological responses in concentrated networks, demonstrating the potential implementation of infrared radiation in breast cancer therapy. ? 2015 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922649527&doi=10.1021%2fpr5011873&partnerID=40&md5=febef72dd46fc9da3c8bc74a76030b5a https://scholars.lib.ntu.edu.tw/handle/123456789/582969 |
ISSN: | 1535-3893 | DOI: | 10.1021/pr5011873 | SDG/關鍵字: | anaphase promoting complex; histone H2AX; integrin; vinculin; actin filament; aminoacylation; Article; breast cancer; breast epithelium cell; cancer cell; cell adhesion; cell communication; cell cycle arrest; cell cycle progression; cell cycle regulation; cell growth; cell invasion; cell metabolism; cell migration; cell migration assay; cell motility; cell proliferation; cell structure; cell viability; colony formation; controlled study; cytoskeleton; DNA content; DNA damage; DNA replication; flow cytometry; focal adhesion; G2 phase cell cycle checkpoint; histone phosphorylation; human; human cell; immunocytochemistry; infrared radiation; liquid chromatography; MCF 7 cell line; microtubule; microtubule organizing center; middle infrared radiation; mitosis; MTT assay; nucleotide metabolism; oxidative stress; priority journal; protein analysis; protein expression; proteomics; purine metabolism; radiation exposure; tandem mass spectrometry; Western blotting; cell division; female; metabolism; pathology; triple negative breast cancer; tumor cell line; Cell Division; Cell Line, Tumor; Chromatography, Liquid; Female; Humans; Infrared Rays; Proteomics; Tandem Mass Spectrometry; Triple Negative Breast Neoplasms |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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