https://scholars.lib.ntu.edu.tw/handle/123456789/586009
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, Ying-Chun | en_US |
dc.contributor.author | Huang, Hao-Ru | en_US |
dc.contributor.author | Hsu, Chia-Hao | en_US |
dc.contributor.author | CHAN-YEN OU | en_US |
dc.date.accessioned | 2021-10-29T06:18:52Z | - |
dc.date.available | 2021-10-29T06:18:52Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 15537390 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/586009 | - |
dc.description.abstract | Neurons are highly specialized cells with polarized cellular processes and subcellular domains. As vital organelles for neuronal functions, mitochondria are distributed by microtubule-based transport systems. Although the essential components of mitochondrial transport including motors and cargo adaptors are identified, it is less clear how mitochondrial distribution among somato-dendritic and axonal compartment is regulated. Here, we systematically study mitochondrial motors, including four kinesins, KIF5, KIF17, KIF1, KLP-6, and dynein, and transport regulators in C. elegans PVD neurons. Among all these motors, we found that mitochondrial export from soma to neurites is mainly mediated by KIF5/UNC-116. Interestingly, UNC-116 is especially important for axonal mitochondria, while dynein removes mitochondria from all plus-end dendrites and the axon. We surprisingly found one mitochondrial transport regulator for minus-end dendritic compartment, TRAK-1, and two mitochondrial transport regulators for axonal compartment, CRMP/UNC-33 and JIP3/UNC-16. While JIP3/UNC-16 suppresses axonal mitochondria, CRMP/UNC-33 is critical for axonal mitochondria; nearly no axonal mitochondria present in unc-33 mutants. We showed that UNC-33 is essential for organizing the population of UNC-116-associated microtubule bundles, which are tracks for mitochondrial trafficking. Disarrangement of these tracks impedes mitochondrial transport to the axon. In summary, we identified a compartment-specific transport regulation of mitochondria by UNC-33 through organizing microtubule tracks for different kinesin motors other than microtubule polarity. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | PLoS genetics | en_US |
dc.title | CRMP/UNC-33 organizes microtubule bundles for KIF5-mediated mitochondrial distribution to axon | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1371/journal.pgen.1009360 | - |
dc.identifier.pmid | 33571181 | - |
dc.identifier.scopus | 2-s2.0-85102322170 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85102322170 | - |
dc.relation.journalvolume | 17 | en_US |
dc.relation.journalissue | 2 | en_US |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.orcid | 0000-0002-6627-5088 | - |
crisitem.author.parentorg | College of Medicine | - |
Appears in Collections: | 生物化學暨分子生物學科研究所 |
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