https://scholars.lib.ntu.edu.tw/handle/123456789/586929
標題: | Aldosterone Excess Induced Mitochondria Decrease and Dysfunction via Mineralocorticoid Receptor and Oxidative Stress In Vitro and In Vivo | 作者: | CHENG-HSUAN TSAI CHIEN-TING PAN Chang, Yi-Yao Peng, Shih-Yuan Lee, Po-Chin CHE-WEI LIAO CHIA-TUNG SHUN Li, Po-Ting VIN-CENT WU Chou, Chia-Hung I-JUNG TSAI CHI-SHENG HUNG YEN-HUNG LIN |
關鍵字: | aldosterone; heart failure; mitochondrial dysfunction; oxidative stress; primary aldosteronism | 公開日期: | 2-八月-2021 | 卷: | 9 | 期: | 8 | 來源出版物: | Biomedicines | 摘要: | Aldosterone excess plays a major role in the progression of cardiac dysfunction and remodeling in clinical diseases such as primary aldosteronism and heart failure. However, the effect of aldosterone excess on cardiac mitochondria is unclear. In this study, we investigated the effect of aldosterone excess on cardiac mitochondrial dysfunction and its mechanisms in vitro and in vivo. We used H9c2 cardiomyocytes to investigate the effect and mechanism of aldosterone excess on cardiac mitochondria, and further investigated them in an aldosterone-infused ICR mice model. The results of the cell study showed that aldosterone excess decreased mitochondrial DNA, COX IV and SOD2 protein expressions, and mitochondria ATP production. These effects were abolished or attenuated by treatment with a mineralocorticoid receptor (MR) antagonist and antioxidant. With regard to the signal transduction pathway, aldosterone suppressed cardiac mitochondria through an MR/MAPK/p38/reactive oxygen species pathway. In the mouse model, aldosterone infusion decreased the amount of cardiac mitochondrial DNA and COX IV protein, and the effects were also attenuated by treatment with an MR antagonist and antioxidant. In conclusion, aldosterone excess induced a decrease in mitochondria and mitochondrial dysfunction via MRs and oxidative stress in vitro and in vivo. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/586929 | ISSN: | 2227-9059 | DOI: | 10.3390/biomedicines9080946 |
顯示於: | 法醫學科所 |
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