https://scholars.lib.ntu.edu.tw/handle/123456789/588857
標題: | Evaluation of macrolide resistance and enhanced molecular typing of Treponema pallidum in patients with syphilis in taiwan: A prospective multicenter study | 作者: | Wu H. SUI-YUAN CHANG Lee N.-Y. Huang W.-C. Wu B.-R. Yang C.-J. Liang S.-H. Lee C.-H. Ko W.-C. Lin H.-H. Chen Y.-H. Liu W.-C. Su Y.-C. Hsieh C.-Y. Wu P.-Y. CHIEN-CHING HUNG |
公開日期: | 2012 | 卷: | 50 | 期: | 7 | 起(迄)頁: | 2299-2304 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | Studies of macrolide resistance mutations and molecular typing using the newly proposed enhanced typing system for Treponema pallidum isolates obtained from HIV-infected patients in the Asia-Pacific region are scarce. Between September 2009 and December 2011, we conducted a survey to detect T. pallidum using a PCR assay using clinical specimens from patients with syphilis at six major designated hospitals for HIV care in Taiwan. The T. pallidum strains were genotyped by following the enhanced molecular typing methodology, which analyzed the number of 60-bp repeats in the acidic repeat protein (arp) gene, T. pallidum repeat (tpr) polymorphism, and the sequence of base pairs 131 to 215 in the tp0548 open reading frame of T. pallidum. Detection of A2058G and A2059G point mutations in the T. pallidum 23S rRNA was performed with the use of restriction fragment length polymorphism (RFLP). During the 2-year study period, 211 clinical specimens were obtained from 136 patients with syphilis. T. pallidum DNA was isolated from 105 (49.8%) of the specimens, with swab specimens obtained from chancres having the highest yield rate (63.2%), followed by plasma (49.4%), serum (35.7%), and cerebrospinal fluid or vitreous fluid (18.2%) specimens. Among the 40 fully typed specimens, 11 subtypes of T. pallidum were identified. Subtype 14f/f (18 isolates) was the most common isolates, followed by 14f/c (3), 14b/c (3), and 14k/f (3). Among the isolates examined for macrolide resistance, none had the A2058G or A2059G mutation. In conclusion, we found that type 14 f/f was the most common T. pallidum strain in this multicenter study on syphilis in Taiwan and that none of the isolates exhibited 23S rRNA mutations causing resistance to macrolides. Copyright ? 2012, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862747290&doi=10.1128%2fJCM.00341-12&partnerID=40&md5=a55545e894c0d8e58894509ce679e43c https://scholars.lib.ntu.edu.tw/handle/123456789/588857 |
ISSN: | 0095-1137 | DOI: | 10.1128/JCM.00341-12 | SDG/關鍵字: | bacterial DNA; macrolide; RNA 23S; adult; antibiotic resistance; antibody titer; article; bacterial gene; bacterial strain; bacterium identification; bacterium isolate; CD4 lymphocyte count; cerebrospinal fluid; controlled clinical trial; controlled study; disease severity; female; gene arp; gene sequence; genetic polymorphism; human; Human immunodeficiency virus infection; major clinical study; male; molecular epidemiology; molecular typing; multicenter study; neurosyphilis; open reading frame; point mutation; polymerase chain reaction; priority journal; prospective study; restriction fragment length polymorphism; syphilis; Taiwan; tpr gene; Treponema pallidum; uveitis; vitreous body; Adult; Anti-Bacterial Agents; Cluster Analysis; DNA, Bacterial; Drug Resistance, Bacterial; Genotype; HIV Infections; Humans; Macrolides; Male; Middle Aged; Molecular Epidemiology; Molecular Typing; Open Reading Frames; Point Mutation; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Prospective Studies; RNA, Ribosomal, 23S; Syphilis; Taiwan; Treponema pallidum |
顯示於: | 醫學系 |
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