https://scholars.lib.ntu.edu.tw/handle/123456789/589275
標題: | Assessment of Candida glabrata strain relatedness by pulsed-field gel electrophoresis and multilocus sequence typing | 作者: | Lin C.-Y. YEE-CHUN CHEN Lo H.-J. Chen K.-W. Li S.-Y. |
公開日期: | 2007 | 卷: | 45 | 期: | 8 | 起(迄)頁: | 2452-2459 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status. Copyright ? 2007, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-34548078121&doi=10.1128%2fJCM.00699-07&partnerID=40&md5=17c79f61b22b022f4d1344bd5b60e8f3 https://scholars.lib.ntu.edu.tw/handle/123456789/589275 |
ISSN: | 0095-1137 | DOI: | 10.1128/JCM.00699-07 | SDG/關鍵字: | amphotericin B; fluconazole; voriconazole; antibiotic sensitivity; article; Candida glabrata; clinical article; clonal variation; DNA extraction; fungus isolation; human; Human immunodeficiency virus infected patient; intensive care unit; mating type; minimum inhibitory concentration; multilocus sequence typing; phylogenetic tree; priority journal; pulsed field gel electrophoresis; statistical analysis; strain difference; Taiwan; teaching hospital; Candida glabrata; Candidiasis; Cluster Analysis; Electrophoresis, Gel, Pulsed-Field; Genes, Mating Type, Fungal; Genotype; Humans; Molecular Epidemiology; Mycological Typing Techniques; Sequence Analysis, DNA; Candida glabrata; Human immunodeficiency virus |
顯示於: | 醫學系 |
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