https://scholars.lib.ntu.edu.tw/handle/123456789/590448
標題: | Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke | 作者: | Lien L.-M. Chiou H.-Y. Yeh H.-L. Chiu S.-Y. JIANN-SHING JENG Lin H.-J. Hu C.-J. Hsieh F.-I. Wei Y.-H. |
公開日期: | 2017 | 卷: | 6 | 期: | 11 | 起(迄)頁: | e006157 | 來源出版物: | Journal of the American Heart Association | 摘要: | Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke. ? 2017 The Authors. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85034790857&doi=10.1161%2fJAHA.117.006157&partnerID=40&md5=62e0c85590e6d70b5375e56bb6540883 https://scholars.lib.ntu.edu.tw/handle/123456789/590448 |
ISSN: | 20479980 | DOI: | 10.1161/JAHA.117.006157 | SDG/關鍵字: | 8 hydroxydeoxyguanosine; glutathione; glutathione disulfide; mitochondrial DNA; biological marker; mitochondrial DNA; adult; Article; brain ischemia; case control study; controlled study; diabetes mellitus; disease association; DNA content; enzyme linked immunosorbent assay; female; human; human cell; leukocyte; major clinical study; male; metabolic syndrome X; oxidative stress; priority journal; quantitative analysis; real time polymerase chain reaction; risk factor; smoking; blood; brain ischemia; clinical trial; genetics; leukocyte; metabolism; middle aged; mitochondrion; multicenter study; nuclear magnetic resonance imaging; oxidative stress; x-ray computed tomography; Biomarkers; Brain Ischemia; Case-Control Studies; DNA, Mitochondrial; Enzyme-Linked Immunosorbent Assay; Female; Humans; Leukocytes; Magnetic Resonance Imaging; Male; Middle Aged; Mitochondria; Oxidative Stress; Real-Time Polymerase Chain Reaction; Tomography, X-Ray Computed |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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