https://scholars.lib.ntu.edu.tw/handle/123456789/593645
標題: | An Evolutionary Landscape of A-to-I RNA Editome across Metazoan Species | 作者: | Li-Yuan Hung Yen-Ju Chen Te-Lun Mai Chia-Ying Chen Min-Yu Yang Tai-Wei Chiang Yi-Da Wang Trees-Juen Chuang |
關鍵字: | A-to-I RNA editing; ADAR; ADAR motif; dynamic editome; evolution | 公開日期: | 2017 | 出版社: | Oxford University Press ({OUP}) | 卷: | 10 | 期: | 2 | 起(迄)頁: | 521-537 | 來源出版物: | Genome Biology and Evolution | 摘要: | Adenosine-to-inosine (A-to-I) editing is widespread across the kingdom Metazoa. However, for the lack of comprehensive analysis in nonmodel animals, the evolutionary history of A-to-I editing remains largely unexplored. Here, we detect high-confidence editing sites using clustering and conservation strategies based on RNA sequencing data alone, without using single-nucleotide polymorphism information or genome sequencing data from the same sample. We thereby unveil the first evolutionary landscape of A-to-I editing maps across 20 metazoan species (from worm to human), providing unprecedented evidence on how the editing mechanism gradually expands its territory and increases its influence along the history of evolution. Our result revealed that highly clustered and conserved editing sites tended to have a higher editing level and a higher magnitude of the ADAR motif. The ratio of the frequencies of nonsynonymous editing to that of synonymous editing remarkably increased with increasing the conservation level of A-to-I editing. These results thus suggest potentially functional benefit of highly clustered and conserved editing sites. In addition, spatiotemporal dynamics analyses reveal a conserved enrichment of editing and ADAR expression in the central nervous system throughout more than 300 Myr of divergent evolution in complex animals and the comparability of editing patterns between invertebrates and between vertebrates during development. This study provides evolutionary and dynamic aspects of A-to-I editome across metazoan species, expanding this important but understudied class of nongenomically encoded events for comprehensive characterization. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/593645 | ISSN: | 1759-6653 | DOI: | 10.1093/gbe/evx277 |
顯示於: | 生命科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。