https://scholars.lib.ntu.edu.tw/handle/123456789/594016
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | KO-JEN LI | en_US |
dc.contributor.author | Lu, Ming-Chi | en_US |
dc.contributor.author | SONG-CHOU HSIEH | en_US |
dc.contributor.author | CHENG-HAN WU | en_US |
dc.contributor.author | Yu, Hsin-Su | en_US |
dc.contributor.author | Tsai, Chang-Youh | en_US |
dc.contributor.author | CHIA-LI YU | en_US |
dc.date.accessioned | 2022-02-10T06:31:13Z | - |
dc.date.available | 2022-02-10T06:31:13Z | - |
dc.date.issued | 2006-08 | - |
dc.identifier.issn | 0741-5400 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/594016 | - |
dc.description.abstract | It is conceivable that a membrane component(s) is transferred from antigen-presenting cells to T cells after antigenic stimulation. However, it is not clear whether a certain membrane component(s) is transferred from polymorphonuclear neturophils (PMN) to T cells for immunomodulation. In the presence study, we cocultured two of the three autologous cells-PMN, CD4+ T, and red blood cells (RBC)-homotypically or heterotypically for 1 h. Spontaneous membrane exchange between autologous PMN-PMN and PMN-CD4+ T but not between CD4+ T-CD4+ T or RBC-CD4+ T was observed with a confocal microscope. Loss of membrane exchange between two paraformaldehyde-fixed cells suggests that mutual membrane exchange is via cell-cell contact. Different combinations of cellular enzyme-linked immunosorbent assay for measuring the binding between fixed cells and biotinylated cell lysates showed the same tendency. To identify the molecule(s) mediating PMN-CD4+ T binding, we compared the banding of biotinylated PMN lysates and the banding of plain PMN lysate probed by biotinylated CD4+ T lysate in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We found that a 75- to 80-kDa surface-expressed molecule on PMN exists constantly to mediate PMN-CD4+ T binding. Peptide analysis disclosed that the molecule had 99.8% identity with lactoferrin (LF). The expression of LF on system lupus erythematosis (SLE)-PMN is less than normal PMN. PMN-CD4+ T coculture increased LF expression on CD4+ T. Normal PMN and human milk-derived LF suppressed interferon-gamma (IFN-gamma) but enhanced interleukin (IL)-10 production of anti-CD3+anti-CD28-activated, normal CD4+ T. In contrast, coculture of SLE-PMN and autologous CD4+ T suppressed IFN-gamma and IL-10 production. These results suggest that the surface-expressed LF released from PMN after contact with autologous CD4+ T modulated its T helper cell type 1 (Th1)/Th2 cytokine production. Decreased LF expression on SLE-PMN abnormally modulates Th1/Th2 production by CD4+ T cells. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY | en_US |
dc.relation.ispartof | Journal of leukocyte biology | en_US |
dc.subject | systemic lupus erythematosus; interferon-gamma; interleukin-10; DELAYED-TYPE HYPERSENSITIVITY; INVIVO IMMUNE-RESPONSE; RED-BLOOD-CELLS; MHC CLASS-II; SELECTIVE DEPLETION; MONOCLONAL-ANTIBODY; T-CELLS; RP-3 TREATMENT; INTERCELLULAR TRANSFER; RHEUMATOID-ARTHRITIS | en_US |
dc.title | Release of surface-expressed lactoferrin from polymorphonuclear neutrophils after contact with CD4+ T cells and its modulation on Th1/Th2 cytokine production | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1189/jlb.1105668 | - |
dc.identifier.pmid | 16769765 | - |
dc.identifier.scopus | 2-s2.0-33748878225 | - |
dc.identifier.isi | WOS:000243015600016 | - |
dc.identifier.url | https://scholars.lib.ntu.edu.tw/handle/123456789/542216 | - |
dc.relation.pages | 350 | en_US |
dc.relation.journalvolume | 80 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pageend | 358 | en_US |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Molecular Medicine | - |
crisitem.author.orcid | 0000-0002-4544-4620 | - |
crisitem.author.orcid | 0000-0002-1513-8657 | - |
crisitem.author.orcid | 0000-0003-4988-0400 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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