https://scholars.lib.ntu.edu.tw/handle/123456789/596346
標題: | Nucleolin antisense oligodeoxynucleotides induce apoptosis and may be used as a potential drug for nasopharyngeal carcinoma therapy | 作者: | Wu C.-D. Chou H.-W. Kuo Y.-S. Lu R.-M. Hwang Y.-C. Wu H.-C. CHIN-TARNG LIN |
關鍵字: | Anti-sense oligodeoxynucleotides; Apoptosis; Nasopharyngeal carcinoma; Nucleolin | 公開日期: | 2012 | 卷: | 27 | 期: | 1 | 起(迄)頁: | 94-100 | 來源出版物: | Oncology Reports | 摘要: | Nucleolin (C23, NCL) mRNA was up-regulated in nasopharyngeal carcinoma (NPC) cells compared to that of normal nasomucosal (NNM) cells using a cDNA microarray approach. The level of nucleolin protein was also up-regulated in 13 NPC cell lines, 30 biopsy specimens and nine other cancer cell lines compared to five NNM cells or normal stromal cells, which were analyzed using immunoblotting or immunohistochemistry. We transfected nucleolin antisense oligodeoxynucleotides (phosphorothioate-modified oligodeoxynucleotides; S-ODNs) into NPC-TW01 cells to knockdown nucleolin expression to evaluate the function of nucleolin in cancer cells. Nucleolin knockdown induced NPC cells but not NNM cells to undergo apoptosis. Furthermore, treatment of NPC-TW01 xenograft tumors with nucleolin antisense oligodeoxynucleotides suppressed the growth of xenograft tumors without obvious side effects. Therefore, we suggest that nucleolin may be a potential cancer therapeutic target and that nucleolin antisense oligodeoxynucleotides may be used as a potential drug for therapy in NPC. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855276410&doi=10.3892%2for.2011.1476&partnerID=40&md5=8b097de75aacc9466524a85429c7a4a2 https://scholars.lib.ntu.edu.tw/handle/123456789/596346 |
ISSN: | 1021-335X | DOI: | 10.3892/or.2011.1476 | SDG/關鍵字: | antisense oligodeoxynucleotide; messenger RNA; nucleolin; nucleolin antisense oligodeoxynucleotide; unclassified drug; animal cell; animal experiment; animal model; antisense therapy; apoptosis; article; cancer cell culture; cancer inhibition; cell viability; controlled study; female; gene expression regulation; gene silencing; genetic transfection; histopathology; human; human cell; immunoblotting; immunohistochemistry; male; mouse; nasopharynx carcinoma; nonhuman; priority journal; protein expression; stroma cell; tumor xenograft; Animals; Antineoplastic Agents; Apoptosis; Carcinoma; Cell Line, Tumor; Female; Gene Knockdown Techniques; Humans; Immunoblotting; Immunohistochemistry; In Situ Nick-End Labeling; Mice; Mice, SCID; Nasopharyngeal Neoplasms; Oligodeoxyribonucleotides, Antisense; Phosphoproteins; Real-Time Polymerase Chain Reaction; RNA, Messenger; RNA-Binding Proteins; Transfection; Xenograft Model Antitumor Assays |
顯示於: | 病理學科所 |
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