https://scholars.lib.ntu.edu.tw/handle/123456789/596359
標題: | Generation and characterization of monoclonal antibodies against dengue virus type 1 for epitope mapping and serological detection by epitope-based peptide antigens | 作者: | Chen Y.-C. Huang H.-N. CHIN-TARNG LIN Chen Y.-F. King C.-C. Wu H.-C. |
公開日期: | 2007 | 卷: | 14 | 期: | 4 | 起(迄)頁: | 404-411 | 來源出版物: | Clinical and Vaccine Immunology | 摘要: | Dengue virus (DEN), the pathogen behind dengue hemorrhagic fever, remains a public health problem in Asia and South America. In this study, monoclonal antibodies (MAbs) against DEN serotype 1 (DEN-1) were generated by fusing NSI/1-Ag4-1 mouse myeloma cells with lymphocytes from BALB/c mice immunized with DEN-1. Twelve MAbs were found to react specifically to the DENs by enzyme-linked immunosorbent assay, immunofluorescence analysis, and immunoblotting analysis. Five MAbs, namely, DA4-7, DA6-7, DA9-5, DA10-2, and DA11-13, were found to react with envelope proteins of DEN-1. Two serotype-specific MAbs of DEN-1, DA6-7 and DA11-13, were further shown to neutralize DEN-1 infection by a plaque reduction neutralization test. The neutralizing epitopes of these MAbs were further identified from a random peptide library displayed on phage. Immunopositive phage clones reacted specifically with these MAbs and did not react with normal mouse serum. Epitope-based peptide antigens were proved able to detect antibodies in serum samples collected from DEN-1-infected patients but not in those taken from DEN-2-infected patients or healthy controls. We believe that these MAbs and neutralizing epitopes will provide information that will lead to the development of DEN-1 serotype-specific diagnostic reagents and vaccines. Copyright ? 2007, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-34248346745&doi=10.1128%2fCVI.00249-06&partnerID=40&md5=32449c385da296f428a33e95730553ee https://scholars.lib.ntu.edu.tw/handle/123456789/596359 |
ISSN: | 1556-6811 | DOI: | 10.1128/CVI.00249-06 | SDG/關鍵字: | antigen; monoclonal antibody; monoclonal antibody da10 2; monoclonal antibody da11 13; monoclonal antibody da4 7; monoclonal antibody da6 7; monoclonal antibody da9 5; unclassified drug; animal cell; animal experiment; article; controlled study; dengue; Dengue virus; drug mechanism; epitope mapping; female; human; mouse; nonhuman; pathogenesis; priority journal; serology; virus strain; Animals; Antibodies, Monoclonal; Antigens, Viral; Cell Line; Dengue Virus; Epitope Mapping; Humans; Mice; Peptides |
顯示於: | 病理學科所 |
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