https://scholars.lib.ntu.edu.tw/handle/123456789/596364
標題: | Disease-specific B cell epitopes for serum antibodies from patients with Severe Acute Respiratory Syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens | 作者: | Liu I.-J. PO-REN HSUEH CHIN-TARNG LIN Chiu C.-Y. CHUAN-LIANG KAO Liao M.-Y. Wu H.-C. |
公開日期: | 2004 | 卷: | 190 | 期: | 4 | 起(迄)頁: | 797-809 | 來源出版物: | Journal of Infectious Diseases | 摘要: | Severe acute respiratory syndrome (SARS) has emerged as a highly contagious, sometimes fatal disease. To find disease-specific B cell epitopes, phage-displayed random peptide libraries were panned on serum immunoglobulin (Ig) G antibodies from patients with SARS. Forty-nine immunopositive phage clones that bound specifically to serum from patients with SARS were selected. These phageborne peptides had 4 consensus motifs, of which 2 corresponded to amino acid sequences reported for SARS-associated coronavirus (SARS-CoV). Synthetic peptide binding and competitive-inhibition assays further confirmed that patients with SARS generated antibodies against SARS-CoV. Immunopositive phage clones and epitope-based peptide antigens demonstrated clinical diagnostic potential by reacting with serum from patients with SARS. Antibody-response kinetics were evaluated in 4 patients with SARS, and production of IgM, IgG, and IgA were documented as part of the immune response. In conclusion, B cell epitopes of SARS corresponded to novel coronavirus. Our epitope-based serologic test may be useful in laboratory detection of the virus and in further study of the pathogenesis of SARS. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-3943049912&doi=10.1086%2f422753&partnerID=40&md5=7985ce762927f5ef2507035658969e54 https://scholars.lib.ntu.edu.tw/handle/123456789/596364 |
ISSN: | 0022-1899 | DOI: | 10.1086/422753 | SDG/關鍵字: | epitope; immunoglobulin A; immunoglobulin G; immunoglobulin G antibody; immunoglobulin M; peptide library; virus antibody; amino acid sequence; antibody blood level; antibody detection; antibody production; antibody response; antigen binding; article; B lymphocyte; binding assay; blood examination; clinical article; clone; competitive inhibition; concentration response; controlled study; human; human cell; immune response; immunogenicity; immunoglobulin production; nucleotide sequence; peptide analysis; phage display; priority journal; SARS coronavirus; severe acute respiratory syndrome; Amino Acid Motifs; Amino Acid Sequence; Animals; Antibodies, Viral; Antigen-Antibody Reactions; Antigens, Viral; B-Lymphocytes; Cercopithecus aethiops; Epitopes; Humans; Molecular Sequence Data; Peptide Library; Peptides; SARS Virus; Sequence Alignment; Severe Acute Respiratory Syndrome; Species Specificity; Vero Cells |
顯示於: | 病理學科所 |
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