https://scholars.lib.ntu.edu.tw/handle/123456789/596783
Title: | Dilute-and-shoot enhances sensitivity of phthalate urinary concentrations for assessing the exposure in children | Authors: | Lien G.-W. Chen J.-H. Tien F.-W. PAU-CHUNG CHEN HUEI-WEN CHEN HSIAO-LIN HWA CHENG-CHUNG FANG TE-I WENG PAI-SHAN CHEN |
Issue Date: | 2018 | Publisher: | Elsevier B.V. | Journal Volume: | 351 | Start page/Pages: | 124-130 | Source: | Journal of Hazardous Materials | Abstract: | Phthalates are not covalently bound to plastics. They can leach from experimental plastic devices. Due to the development of sensitive techniques, exogenous sources that interfere with the accuracy of phthalate monoester analysis can be easily detected. Here, we propose the simple and rapid dilute-and-shoot method to minimize sample handling and limit contact with laboratory apparatus, which efficiently reduced phthalate interferences mainly from experimental plasticware and improved the accuracy of analysis. Chemical additives in the mobile phase and modifiers in reconstituted solution were evaluated to improve peak shape and liquid chromatography separation. Under optimal conditions, the limit of detection ranged from 0.01 to 0.5 ng/mL. No phthalate monoesters were detected above the limit of detection in blank water while the leached concentrations of MMP, MEP, MiBP, MnBP and MEHP from the conventional solid phase extraction procedure were 1.9, 2.7, 0.9, 2.0 and 1.1 ng/mL, respectively. In 304 samples collected from primary school– children aged 9–10 years, we observed a positive association between body mass index (BMI) and urinary MMP and MiBP concentrations in boys, while MEOHP and MEHHP were inversely related to BMI in girls. Furthermore, detectable phthalate monoesters may be considered potential chemical obesogens related to BMI in children. ? 2018 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043308888&doi=10.1016%2fj.jhazmat.2018.02.050&partnerID=40&md5=616d72d869d8080d4e652ca43b632760 https://scholars.lib.ntu.edu.tw/handle/123456789/596783 |
ISSN: | 0304-3894 | DOI: | 10.1016/j.jhazmat.2018.02.050 | SDG/Keyword: | Chromatography; Health; Leaching; Liquid chromatography; Manganese compounds; Mass spectrometry; Phase separation; Body mass index; Dilute-and-shoot; Liquid chromatography - mass spectrometries; Phthalates; Urine; Esters; acetic acid; ammonium acetate; deionized water; mono (2 ethyl 5 hydroxyhexyl)phthalate; mono (2 ethyl 5 oxohexyl)phthalate; mono (2 ethylhexyl)phthalate; mono (3 carboxypropyl)phthalate; mono cyclohexyl phthalate; mono ethyl phthalate; mono isobutyl phthalate; mono isononyl phthalate; mono methyl phthalate; mono n butyl phthalate; mono n octyl phthalate; monobenzyl phthalate; phthalic acid derivative; unclassified drug; phthalic acid derivative; accuracy assessment; age class; body mass; chemical method; child health; concentration (composition); detection method; extraction method; liquid chromatography; mass spectrometry; phthalate; plastic; pollution exposure; sensitivity analysis; urine; Article; body mass; child; dilute and shoot method; electrospray mass spectrometry; exposure; female; human; laboratory test; limit of detection; limit of quantitation; liquid chromatography-mass spectrometry; major clinical study; male; measurement accuracy; primary school; school child; sensitivity analysis; sex difference; solid phase extraction; urine level; urine sampling; environmental exposure; pollutant; specimen handling; urine; Body Mass Index; Child; Environmental Exposure; Environmental Pollutants; Female; Humans; Male; Phthalic Acids; Specimen Handling |
Appears in Collections: | 毒理學研究所 |
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