https://scholars.lib.ntu.edu.tw/handle/123456789/596848
標題: | Snail regulates Nanog status during the epithelial-mesenchymal transition via the Smad1/Akt/GSK3β signaling pathway in non-small-cell lung cancer | 作者: | Liu C.-W. Li C.-H. Peng Y.-J. Cheng Y.-W. HUEI-WEN CHEN Liao P.-L. Kang J.-J. Yeng M.-H. |
關鍵字: | Akt; Cancer stem cell; Epithelial-mesenchymal transition; Nanog; Noggin; Non-small-cell lung cancer; Smad; Snail | 公開日期: | 2014 | 出版社: | Impact Journals LLC | 卷: | 5 | 期: | 11 | 起(迄)頁: | 3880-3894 | 來源出版物: | Oncotarget | 摘要: | The epithelial-mesenchymal transition (EMT), a crucial step in cancer metastasis, is important in transformed cancer cells with stem cell-like properties. In this study, we established a Snail-overexpressing cell model for non-small-cell lung cancer (NSCLC) and investigated its underlying mechanism. We also identified the downstream molecular signaling pathway that contributes to the role of Snail in regulating Nanog expression. Our data shows that high levels of Snail expression correlate with metastasis and high levels of Nanog expression in NSCLC. NSCLC cells expressing Snail are characterized by active EMT characteristics and exhibit an increased ability to migrate, chemoresistance, sphere formation, and stem cell-like properties. We also investigated the signals required for Snail-mediated Nanog expression. Our data demonstrate that LY294002, SB431542, LDN193189, and Noggin pretreatment inhibit Snail-induced Nanog expression during EMT. This study shows a significant correlation between Snail expression and phosphorylation of Smad1, Akt, and GSK3β. In addition, pretreatment with SB431542, LDN193189, or Noggin prevented Snail-induced Smad1 and Akt hyperactivation and reactivated GSK3β. Moreover, LY294002 pretreatment prevented Akt hyperactivation and reactivated GSK3β without altering Smad1 activation. These findings provide a novel mechanistic insight into the important role of Snail in NSCLC during EMT and indicate potentially useful therapeutic targets for NSCLC. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903540246&doi=10.18632%2foncotarget.2006&partnerID=40&md5=2500fc16e529c1114f863ca2492af1e6 https://scholars.lib.ntu.edu.tw/handle/123456789/596848 |
ISSN: | 1949-2553 | DOI: | 10.18632/oncotarget.2006 | SDG/關鍵字: | 2 morpholino 8 phenylchromone; 4 [4 (1,3 benzodioxol 5 yl) 5 (2 pyridinyl) 1h imidazol 2 yl]benzamide; glycogen synthase kinase 3beta; noggin; protein kinase B; Smad1 protein; transcription factor NANOG; transcription factor Snail; glycogen synthase kinase 3; glycogen synthase kinase 3 beta; homeodomain protein; NANOG protein, human; protein kinase B; Smad1 protein; SMAD1 protein, human; snail family transcription factors; transcription factor; animal experiment; animal model; animal tissue; article; cancer resistance; cancer stem cell; carcinogenicity; controlled study; enzyme activation; enzyme reactivation; epithelial mesenchymal transition; female; human; human cell; human tissue; in vivo study; lung non small cell cancer; major clinical study; male; metastasis; mouse; nonhuman; protein expression; protein function; protein phosphorylation; signal transduction; tumor biopsy; animal; antagonists and inhibitors; Bagg albino mouse; epithelial mesenchymal transition; genetic transfection; lung tumor; metabolism; middle aged; non small cell lung cancer; pathology; physiology; SCID mouse; xenograft; Animals; Carcinoma, Non-Small-Cell Lung; Epithelial-Mesenchymal Transition; Female; Glycogen Synthase Kinase 3; Heterografts; Homeodomain Proteins; Humans; Lung Neoplasms; Male; Mice; Mice, Inbred BALB C; Mice, SCID; Middle Aged; Neoplastic Stem Cells; Proto-Oncogene Proteins c-akt; Signal Transduction; Smad1 Protein; Transcription Factors; Transfection |
顯示於: | 毒理學研究所 |
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