https://scholars.lib.ntu.edu.tw/handle/123456789/597567
標題: | Kisspeptin modulates fertilization capacity of mouse spermatozoa | 作者: | Hsu, Meng-Chieh Wang, Jyun-Yuan Lee, Yue-Jia Jong, De-Shien Tsui, Kuan-Hao DE-SHIEN JONG CHIH-HSIEN CHIU YUE-JIA LEE MENG-CHIEH HSU |
關鍵字: | METASTASIS SUPPRESSOR GENE; PROTEIN-COUPLED RECEPTOR; ADULT MALE RATS; ACROSOME REACTION; TYROSINE PHOSPHORYLATION; TESTICULAR DEGENERATION; HORMONE NEURONS; KISS-1; SPERM; GPR54 | 公開日期: | 六月-2014 | 出版社: | BIOSCIENTIFICA LTD | 卷: | 147 | 期: | 6 | 起(迄)頁: | 835 | 來源出版物: | Reproduction (Cambridge, England) | 摘要: | Kisspeptin acts as an upstream regulator of the hypothalamus-pituitary-gonad axis, which is one of the main regulatory systems for mammalian reproduction. Kiss1 and its receptor Kiss1r (also known as G protein-coupled receptor 54 (Gpr54)) are expressed in various organs, but their functions are not well understood. The purpose of this study was to investigate the expression profiles and functions of kisspeptin and KISS1R in the reproductive tissues of imprinting control region mice. To identify the expression pattern and location of kisspeptin and KISS1R in gonads, testes and ovarian tissues were examined by immunohistochemical or immunofluorescent staining. Kisspeptin and KISS1R were expressed primarily in Leydig cells and seminiferous tubules respectively. KISS1R was specifically localized in the acrosomal region of spermatids and mature spermatozoa. Kisspeptin, but not KISS1R, was expressed in the cumulus-oocyte complex and oviductal epithelium of ovarian and oviductal tissues. The sperm intracellular calcium concentrations significantly increased in response to treatment with kisspeptin 10 in Fluo-4-loaded sperm. The IVF rates decreased after treatment of sperm with the kisspeptin antagonist peptide 234. These results suggest that kisspeptin and KISS1R might be involved in the fertilization process in the female reproductive tract. In summary, this study indicates that kisspeptin and KISS1R are expressed in female and male gametes, respectively, and in mouse reproductive tissues. These data strongly suggest that the kisspeptin system could regulate mammalian fertilization and reproduction. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/597567 | ISSN: | 1470-1626 1741-7899 |
DOI: | 10.1530/REP-13-0368 | SDG/關鍵字: | calcium; chorionic gonadotropin; kisspeptin; kisspeptin 10; kisspeptin receptor 1; seric gonadotropin; unclassified drug; calcium; G protein coupled receptor; Kiss1 protein, mouse; Kiss1r protein, mouse; kisspeptin; adult; animal cell; animal experiment; animal tissue; article; calcium cell level; controlled study; cumulus cell; female; fertilization; fertilization in vitro; gonad; immunodetection; immunofluorescence; immunohistochemistry; Leydig cell; male; molecular imprinting; mouse; nonhuman; nucleotide sequence; oocyte; ovary; oviduct; priority journal; protein expression; protein localization; seminiferous tubule; spermatid; spermatozoon; animal; epididymis; fertility; genetics; Institute for Cancer Research mouse; metabolism; pregnancy; signal transduction; spermatozoon; time; Animals; Calcium; Epididymis; Female; Fertilization; Fertilization in Vitro; Kisspeptins; Leydig Cells; Male; Mice, Inbred ICR; Oocytes; Ovary; Oviducts; Pregnancy; Receptors, G-Protein-Coupled; Seminiferous Tubules; Signal Transduction; Sperm-Ovum Interactions; Spermatozoa; Time Factors |
顯示於: | 動物科學技術學系 |
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