https://scholars.lib.ntu.edu.tw/handle/123456789/598256
標題: | Kinetic and thermodynamic studies of lysozyme adsorption on cibacron blue f3ga dye-ligand immobilized on aminated nanofiber membrane | 作者: | Hsin A How S.-C Wang S.S.-S Ooi C.W Chiu C.-Y Chang Y.-K. STEVEN SHENG-SHIH WANG |
關鍵字: | Adsorption;Dye affinity ligand;Kinetic;Lysozyme;Nanofibrous membrane;Thermodynamic;Adsorption isotherms;Alkalinity;Dyes;Enzymes;Free energy;Ionic strength;Ligands;Membranes;Nanofibers;Affinity ligands;Dye affinity;Dye-ligands;Ethylene diamine;Free energy change;Lysozyme adsorption;Nanofiber membrane;Nanofibrous membranes;Polyacrylonitrile nanofibre;Kinetics | 公開日期: | 2021 | 卷: | 11 | 期: | 12 | 來源出版物: | Membranes | 摘要: | The polyacrylonitrile (PAN) nanofiber membrane was prepared by the electrospinning technique. The nitrile group on the PAN nanofiber surface was oxidized to carboxyl group by alkaline hydrolysis. The carboxylic group on the membrane surface was then converted to dye affinity membrane through reaction with ethylenediamine (EDA) and Cibacron Blue F3GA, sequentially. The adsorption characteristics of lysozyme onto the dye ligand affinity nanofiber membrane (namely P-EDA-Dye) were investigated under various conditions (e.g., adsorption pH, EDA coupling concentration, lysozyme concentration, ionic strength, and temperature). Optimum experimental parameters were determined to be pH 7.5, a coupling concentration of EDA 40 ?mol/mL, and an immobilization density of dye 267.19 mg/g membrane. To understand the mechanism of adsorption and possible rate controlling steps, a pseudo first-order, a pseudo second-order, and the Elovich models were first used to describe the experimental kinetic data. Equilibrium isotherms for the adsorption of lysozyme onto P-EDA-Dye nanofiber membrane were determined experimentally in this work. Our kinetic analysis on the adsorption of lysozyme onto P-EDA-Dye nanofiber membranes revealed that the pseudo second-order rate equation was favorable. The experimental data were satisfactorily fitted by the Langmuir isotherm model, and the thermodynamic parameters including the free energy change, enthalpy change, and entropy change of adsorption were also determined accordingly. Our results indicated that the free energy change had a negative value, suggesting that the adsorption process occurred spontaneously. Moreover, after five cycles of reuse, P-EDA-Dye nanofiber membranes still showed promising efficiency of lysozyme adsorption. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121301408&doi=10.3390%2fmembranes11120963&partnerID=40&md5=65280afdc16fc82097255e82f6cd2fbe https://scholars.lib.ntu.edu.tw/handle/123456789/598256 |
ISSN: | 20770375 | DOI: | 10.3390/membranes11120963 |
顯示於: | 化學工程學系 |
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