https://scholars.lib.ntu.edu.tw/handle/123456789/598657
標題: | Therapeutic Potential of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Recovering From Murine Pulmonary Emphysema Under Cigarette Smoke Exposure | 作者: | Chen X.-Y Chen Y.-Y Lin W Chen C.-H Wen Y.-C Hsiao T.-C Chou H.-C Chung K.F Chuang H.-C. TA-CHIH HSIAO |
關鍵字: | cigarette smoke;COPD;emphysema;inflammation;stem cell;CXCL1 chemokine;elastin;fibronectin;interleukin 17;interleukin 1beta;keratinocyte derived chemokine;macrophage elastase;messenger RNA;tumor necrosis factor;animal experiment;animal model;animal tissue;antiinflammatory activity;Article;body weight;bronchoalveolar lavage fluid;C57BL 6 mouse;cell proliferation;cell viability;chronic obstructive lung disease;controlled study;cytokine production;disease severity;human;human cell;lung emphysema;lung fibroblast;lung infiltrate;lymphocyte count;male;mouse;mRNA expression level;nonhuman;protein blood level;treatment outcome;umbilical cord mesenchymal stem cell transplantation | 公開日期: | 2021 | 卷: | 8 | 來源出版物: | Frontiers in Medicine | 摘要: | Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were shown to have potential for immunoregulation and tissue repair. The objective of this study was to investigate the effects of hUC-MSCs on emphysema in chronic obstructive pulmonary disease (COPD). The C57BL/6JNarl mice were exposed to cigarette smoke (CS) for 4 months followed by administration of hUC-MSCs at 3 × 106 (low dose), 1 × 107 (medium dose), and 3 × 107 cells/kg body weight (high dose). The hUC-MSCs caused significant decreases in emphysema severity by measuring the mean linear intercept (MLI) and destructive index (DI). A decrease in neutrophils (%) and an increase in lymphocytes (%) in bronchoalveolar lavage fluid (BALF) were observed in emphysematous mice after hUC-MSC treatment. Lung levels of interleukin (IL)-1β, C-X-C motif chemokine ligand 1 (CXCL1)/keratinocyte chemoattractant (KC), and matrix metalloproteinase (MMP)-12 significantly decreased after hUC-MSC administration. Significant reductions in tumor necrosis factor (TNF)-α, IL-1β, and IL-17A in serum occurred after hUC-MSC administration. Notably, the cell viability of lung fibroblasts improved with hUC-MSCs after being treated with CS extract (CSE). Furthermore, the hUC-MSCs-conditioned medium (hUC-MSCs-CM) restored the contractile force, and increased messenger RNA expressions of elastin and fibronectin by lung fibroblasts. In conclusion, hUC-MSCs reduced inflammatory responses and emphysema severity in CS-induced emphysematous mice. ? Copyright ? 2021 Chen, Chen, Lin, Chen, Wen, Hsiao, Chou, Chung and Chuang. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116884636&doi=10.3389%2ffmed.2021.713824&partnerID=40&md5=bff9c679638865b8db8efcb9595026c9 https://scholars.lib.ntu.edu.tw/handle/123456789/598657 |
ISSN: | 2296858X | DOI: | 10.3389/fmed.2021.713824 |
顯示於: | 環境工程學研究所 |
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