https://scholars.lib.ntu.edu.tw/handle/123456789/598924
標題: | A size reduction method for rapid digital PCR using thin-film chip and vacuum pouch microfluidic system | 作者: | Lee C.-J Hsu Y.-H. YU-HSIANG HSU |
關鍵字: | Digital PCR;Point-of-care testing;Self-digitization;Self-driven microfluidics;Thin-film microfluidic chip;Vacuum pouch microfluidic system;Chip scale packages;Digital microfluidics;Drops;Fluidic devices;Optical systems;Polymer films;Programmable logic controllers;RNA;System-on-chip;Thin films;Digitisation;Microfluidic-chips;Microfluidics systems;Self-driven;Self-driven microfluidic;Thin-films;Polymerase chain reaction | 公開日期: | 2022 | 卷: | 26 | 期: | 1 | 來源出版物: | Microfluidics and Nanofluidics | 摘要: | The digital polymerase chain reaction (dPCR) is a high efficacy detection method that is widely applied in lab-on-a-chip systems. Reported dPCR microfluidic devices usually require a high level of system integration, including a fluidic manipulating system, a thermal cycling system, an optical system, and a power source. To effectively reduce system size and complexity, we integrate thin-film dPCR chip and the vacuum pouch microfluidic (VPM) system to eliminate the fluidic manipulation system and to reduce energy demand on the thermal cycler. The VPM system integrates fluidic handling system on-chip by using a vacuum seal bag to enclose the thin-film dPCR chip and to store negative pressure. This design makes operation simple: load PCR mix and oil on the pouch surface then pierce the film surface to initiate loading and droplet generation. The thin-film dPCR chip is a 0.288?mm-thick cyclo-olefin-polymer film embedded with a passive droplet generator, and the distance from droplets to the chip surface is less than 50?μm. This design makes this chip have a low thermal mass and a short thermal conducting length, and the demand on the heating and cooling power of the thermal cycler can be largely reduced. A polyimide thermofoil heater and a small cooling fan are sufficient to complete 35 amplification cycles in 4.84?min. Combining these two technologies, this VPM–dPCR system is lightweight, compact, and conserves energy, and the dPCR chip is disposable. Finally, its capability to perform dual-target detection also is demonstrated by quantifying two microRNA targets in one VPM–dPCR chip. ? 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121416897&doi=10.1007%2fs10404-021-02507-x&partnerID=40&md5=712a643fca4292051082942543f7e623 https://scholars.lib.ntu.edu.tw/handle/123456789/598924 |
ISSN: | 16134982 | DOI: | 10.1007/s10404-021-02507-x |
顯示於: | 應用力學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。