https://scholars.lib.ntu.edu.tw/handle/123456789/598996
Title: | Modified low-temperature extraction method for isolation of bletilla striata polysaccharide as antioxidant for the prevention of alzheimer’s disease | Authors: | Lin Y.-W Fang C.-H Liang Y.-J Liao H.-H Lin F.-H. Lin, Feng-Huei |
Keywords: | Aluminum chloride;Alzheimer’s disease;Amyloid-β;Anti-inflammation;Bletilla striata;Oxidative stress;aluminum chloride;antiinflammatory agent;antioxidant;polysaccharide;polysaccharide b, Bletilla striata;reactive oxygen metabolite;Alzheimer disease;animal;cold;drug effect;female;metabolism;oxidative stress;pathology;rat;Sprague Dawley rat;Aluminum Chloride;Alzheimer Disease;Animals;Anti-Inflammatory Agents;Antioxidants;Cold Temperature;Female;Oxidative Stress;Polysaccharides;Rats;Rats, Sprague-Dawley;Reactive Oxygen Species | Issue Date: | 2021 | Journal Volume: | 22 | Journal Issue: | 23 | Source: | International Journal of Molecular Sciences | Abstract: | Amyloid-β (Aβ) peptides play a key role in Alzheimer’s disease (AD), the most common type of dementia. In this study, a polysaccharide from Bletilla striata (BSP), with strong antioxidant and anti-inflammatory properties, was extracted using a low-temperature method and tested for its efficacy against AD, in vitro using N2a and BV-2 cells, and in vivo using an AD rat model. The characterization of the extracted BSP for its molecular structure and functional groups demonstrated the effectiveness of the modified method for retaining its bioactivity. In vitro, BSP reduced by 20% reactive oxygen species (ROS) levels in N2a cells (p = 0.0082) and the expression levels of inflammation-related genes by 3-fold TNF-α (p = 0.0048), 4-fold IL-6 (p = 0.0019), and 2.5-fold IL-10 (p = 0.0212) in BV-2 cells treated with Aβ fibrils. In vivo, BSP recovered learning memory, ameliorated morphological damage in the hippocampus and cortex, and reduced the expression of the β-secretase protein in AlCl3-induced AD rats. Collectively, these findings demonstrated the efficacy of BSP for preventing and alleviating the effects of AD. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119931586&doi=10.3390%2fijms222312760&partnerID=40&md5=52285644cf30bb9ffa278fa6ac32c5ec https://scholars.lib.ntu.edu.tw/handle/123456789/598996 |
ISSN: | 16616596 | DOI: | 10.3390/ijms222312760 |
Appears in Collections: | 醫學工程學研究所 |
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