https://scholars.lib.ntu.edu.tw/handle/123456789/600267
標題: | A nanofluidic preconcentrator integrated with an aluminum-based nanoplasmonic sensor for Epstein-Barr virus detection | 作者: | Hsieh H.-Y Luo J.-X Shen Y.-H Lo S.-C Hsu Y.-C Tahara H Fan Y.-J Wei P.-K Sheen H.-J. HORN-JIUNN SHEEN |
關鍵字: | Epstein-Barr virus (EBV);Latent membrane protein 1;Localized surface plasmon resonance;Nanofluidic preconcentration;Aluminum;Aluminum coatings;Coronavirus;Deposition;Diagnosis;Nanoimprint lithography;Nanostructures;Proteins;Epstein-Barr virus;Membrane proteins;Nanoslits;Pre-concentration;Preconcentrators;Sensing chip;Nanofluidics | 公開日期: | 2022 | 卷: | 355 | 來源出版物: | Sensors and Actuators B: Chemical | 摘要: | Epstein-Barr virus (EBV) positivity is one of the indexes for diagnosing nasopharyngeal carcinoma (NPC). Moreover, systemic inflammatory responses can easily be triggered in patients who are both EBV- and coronavirus disease 2019 (COVID-19)-positive. Development of rapid and highly sensitive EBV screening methods has become important. In this study, a nanofluidic preconcentrator integrated with a nanoslit Fano resonance biosensor was developed to detect latent membrane protein 1 (LMP1) for an EBV diagnosis. Through nanoimprinting and aluminum deposition, the low-cost nanoslit plasmonic sensing chip can be mass-produced. The nanoporous membrane was patterned on a sensing chip as an ion selective channel to concentrate LMP1 proteins. Anti-LMP1 immunoglobulin G was then modified to a sensing chip to immunosense LMP1. The Fano resonant spectrum of the capped nanoslit array produced a transmission peak followed by a dip. We recorded and analyzed the spectrum using four methods, including area, center of mass, peak value, and dip value methods. With preconcentration, a limit of detection (LOD) of 100 pg/ml and a sensing range of 100 pg/ml to 10 ?g/ml was achieved using the peak value. ? 2021 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121990430&doi=10.1016%2fj.snb.2021.131327&partnerID=40&md5=2e09b45763c2ebce02bfaec9b7bf6cea https://scholars.lib.ntu.edu.tw/handle/123456789/600267 |
ISSN: | 09254005 | DOI: | 10.1016/j.snb.2021.131327 |
顯示於: | 應用力學研究所 |
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