https://scholars.lib.ntu.edu.tw/handle/123456789/600271
標題: | Nanoplasmonic structure of a polycarbonate substrate integrated with parallel microchannels for label-free multiplex detection | 作者: | Lu Y.-J Hsieh H.-Y Kuo W.-C Wei P.-K Sheen H.-J Tahara H Chiu T.-W Fan Y.-J. HORN-JIUNN SHEEN |
關鍵字: | Biosensor;Multiplex detection;Nanoimprinting;Surface plasmon resonance (SPR);Microchannels;Microfluidics;Plasma applications;Polycarbonates;Polydimethylsiloxane;Refractive index;Silicones;Surface plasmon resonance;Surface treatment;Immunoglobulin G;Localized surface plasmon resonance;Microfluidics channels;Multiplex detections;Nano-imprinting;Polycarbonate substrates;Sensing devices;Surface plasmon resonance sensing;Surface-plasmon resonance;Fluidic devices | 公開日期: | 2021 | 卷: | 13 | 期: | 19 | 來源出版物: | Polymers | 摘要: | In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumi-nation and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116072256&doi=10.3390%2fpolym13193294&partnerID=40&md5=0bde30abe76ca47eb85a0a74224beceb https://scholars.lib.ntu.edu.tw/handle/123456789/600271 |
ISSN: | 20734360 | DOI: | 10.3390/polym13193294 |
顯示於: | 應用力學研究所 |
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