https://scholars.lib.ntu.edu.tw/handle/123456789/600274
標題: | Development of a water refractive index-matched microneedle integrated into a light sheet microscopy system for continuous embryonic cell imaging | 作者: | Fan Y.-J Hsieh H.-Y Huang Y.-R Tsao C Lee C.-M Tahara H Wu Y.-C Sheen H.-J Chen B.-C. HORN-JIUNN SHEEN |
關鍵字: | Incident light;Needles;Photobleaching;Caenorhabditis elegans;Cell imaging;Circular surfaces;Embryonic cells;Incident angles;Light sheet;Light-sheet microscopies;Microneedles;Microscopy systems;Subcellular resolution;Refractive index | 公開日期: | 2022 | 出版社: | Royal Society of Chemistry | 卷: | 22 | 期: | 3 | 起(迄)頁: | 584-591 | 來源出版物: | Lab on a Chip | 摘要: | In this study, microneedle-integrated light sheet microscopy (LSM) was developed for trapping and continuously imaging embryos of Caenorhabditis elegans with subcellular resolution. To reduce aberrations when the light sheet was propagated into the device, a microneedle was fabricated using a transparent, water refractive index-matched polymer. It was proven that when the light sheet emerged from the water-immersed objective and penetrated through the microneedle with a circular surface, even with a non-perpendicular incident angle, fewer aberrations were found. An embryo was injected into and trapped at the tip of the microneedle, which was positioned at the interrogation window of the LSM apparatus with the image plane perpendicular to the light sheet, and this setup was used to sequentially acquire embryo images. By applying the light sheet, higher-resolution, higher-contrast images were obtained. The system also showed low photobleaching and low phototoxicity to embryos of C. elegans. Furthermore, three-dimensional embryo images with a whole field of view of the microneedle could be achieved by stitching together images and reconstructing sequential two-dimensional embryo images. ? The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123968860&doi=10.1039%2fd1lc00827g&partnerID=40&md5=02b1ba1d133639d28f22a5a3932a956e https://scholars.lib.ntu.edu.tw/handle/123456789/600274 |
ISSN: | 14730197 | DOI: | 10.1039/d1lc00827g |
顯示於: | 應用力學研究所 |
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