https://scholars.lib.ntu.edu.tw/handle/123456789/600277
標題: | Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection | 作者: | Sheen H.-J HORN-JIUNN SHEEN |
關鍵字: | Epstein-barr virus;LMP1;Nanoslit;Polymerase chain reaction;Surface plasmon resonance;DNA;Gold;Microfluidics;Nanoimprint lithography;Nanostructures;Surface reactions;Viruses;Epstein-Barr virus;Hot-embossing;Laser scribing;Latent membrane protein 1;Membrane proteins;Nanoslits;Sensing chip;Surface plasmon resonance sensor;Surface-plasmon resonance;Virus detection;gold nanoparticle;latent membrane protein 1;gold;matrix protein;Article;calibration;controlled study;DNA extraction;DNA sequence;Epstein Barr virus;limit of detection;measurement accuracy;microfluidics;nonhuman;polymerase chain reaction;sensitivity analysis;static electricity;surface plasmon resonance;virus detection;Epstein Barr virus infection;genetic procedures;genetics;human;Biosensing Techniques;COVID-19;Epstein-Barr Virus Infections;Herpesvirus 4, Human;Humans;Polymerase Chain Reaction;SARS-CoV-2;Viral Matrix Proteins | 公開日期: | 2022 | 卷: | 195 | 來源出版物: | Biosensors and Bioelectronics | 摘要: | We present the first combination of a microfluidic polymerase chain reaction (PCR) with a gold nanoslit-based surface plasmon resonance (SPR) sensor for detecting the DNA sequence of latent membrane protein 1 (LMP1). The PCR microchannel was produced through a laser scribing technique, and the SPR nanoslit chip was manufactured via hot-embossing nanoimprinting lithography. Afterward, the LMP1 DNA probe was adsorbed onto the SPR chip of the integrated device through electrostatic interactions for further detection. The device can complete the analytical procedure in around 36 min, while the traditional machine requires 105 min to achieve similar signals under the same PCR thermal cycles. The calibration curve with serially diluted LMP1 DNA exhibited the accuracy (R2 > 0.99) and sensitivity (limit of detection: ?10?11 g/mL) of the device. Moreover, extracted DNA from Epstein-Barr virus (EBV)-positive cells were directly detected through the integrated chip. In brief, this all-in-one chip can amplify gene fragments at the front-end and detect them at the back-end, decreasing the time required for the analysis without compromising accuracy or sensitivity. We believe this label-free, real-time, low-cost device has enormous potential for rapid detection of various viruses, such as EBV and COVID-19. ? 2021 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116193793&doi=10.1016%2fj.bios.2021.113672&partnerID=40&md5=967fcc7670b66cb91ec4af7bf2f234e0 https://scholars.lib.ntu.edu.tw/handle/123456789/600277 |
ISSN: | 09565663 | DOI: | 10.1016/j.bios.2021.113672 |
顯示於: | 應用力學研究所 |
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