https://scholars.lib.ntu.edu.tw/handle/123456789/600371
標題: | Anti?inflammatory fibronectin?agnp for regulation of biological performance and endothelial differentiation ability of mesenchymal stem cells | 作者: | Hung H.-S Chang K.-B Tang C.-M Ku T.-R Kung M.-L Yu A.Y.-H Shen C.-C Yang Y.-C Hsieh H.-H SHAN-HUI HSU |
關鍵字: | Endothelial differentiation;Fibronectin;Mesenchymal stem cells;Silver nanoparticles;Vascular tissue engineering;antiinflammatory agent;fibronectin;matrix metalloproteinase;metal nanoparticle;reactive oxygen metabolite;silver;animal;cell culture;cell differentiation;cell proliferation;cytology;cytoskeleton;drug effect;endothelium cell;immunohistochemistry;infrared spectroscopy;mesenchymal stem cell;metabolism;particle size;rat;ultrastructure;Animals;Anti-Inflammatory Agents;Cell Differentiation;Cell Proliferation;Cells, Cultured;Cytoskeleton;Endothelial Cells;Fibronectins;Immunohistochemistry;Matrix Metalloproteinases;Mesenchymal Stem Cells;Metal Nanoparticles;Particle Size;Rats;Reactive Oxygen Species;Silver;Spectroscopy, Fourier Transform Infrared | 公開日期: | 2021 | 卷: | 22 | 期: | 17 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | The engineering of vascular regeneration still involves barriers that need to be conquered. In the current study, a novel nanocomposite comprising of fibronectin (denoted as FN) and a small amount of silver nanoparticles (AgNP, ~15.1, ~30.2 or ~75.5 ppm) was developed and its biological function and biocompatibility in Wharton’s jelly?derived mesenchymal stem cells (MSCs) and rat models was investigated. The surface morphology as well as chemical composition for pure FN and the FN?AgNP nanocomposites incorporating various amounts of AgNP were firstly characterized by atomic force microscopy (AFM), UV?Visible spectroscopy (UV?Vis), and Fourier?transform infrared spectroscopy (FTIR). Among the nanocomposites, FN?AgNP with 30.2 ppm silver nanoparticles demonstrated the best biocompatibility as assessed through intracellular ROS production, proliferation of MSCs, and monocytes activation. The expression levels of pro?inflammatory cytokines, TNF?α, IL?1β, and IL?6, were also examined. FN?AgNP 30.2 ppm significantly inhibited pro?inflammatory cytokine expression compared to other materials, indicating superior performance of anti?immune response. Mechanistically, FN?AgNP 30.2 ppm significantly induced greater expression of vascular endothelial growth factor (VEGF) and stromal?cell derived factor?1 alpha (SDF?1α) and promoted the migration of MSCs through matrix metalloproteinase (MMP) signaling pathway. Besides, in vitro and in vivo studies indicated that FN?AgNP 30.2 ppm stimulated greater protein expressions of CD31 and von Willebrand Factor (vWF) as well as facilitated better endothelialization capacity than other materials. Furthermore, the histological tissue examination revealed the lowest capsule formation and collagen deposition in rat subcutaneous implantation of FN? AgNP 30.2 ppm. In conclusion, FN?AgNP nanocomposites may facilitate the migration and proliferation of MSCs, induce endothelial cell differentiation, and attenuate immune response. These finding also suggests that FN?AgNP may be a potential anti?inflammatory surface modification strategy for vascular biomaterials. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113466909&doi=10.3390%2fijms22179262&partnerID=40&md5=dd025605dd1ce0a82ffd7974b887ea1c https://scholars.lib.ntu.edu.tw/handle/123456789/600371 |
ISSN: | 16616596 | DOI: | 10.3390/ijms22179262 |
顯示於: | 高分子科學與工程學研究所 |
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