https://scholars.lib.ntu.edu.tw/handle/123456789/604928
標題: | Identification of genes with consistent methylation levels across different human tissues | 作者: | TZU-PIN LU TZE-HSIANG CHEN Tsai, M.-H. KUAN-TING KUO CHUHSING KATE HSIAO LIANG-CHUAN LAI ERIC YAO-YU CHUANG |
公開日期: | 2014 | 出版社: | Nature Publishing Groups | 卷: | 4 | 來源出版物: | Scientific Reports | 摘要: | DNA methylation plays an important role in regulating cell growth and disease development. Methylation profiles are examined by bisulfite conversion; however, the lack of markers for bisulfite conversion efficiency and appropriate internal control genes remains a major challenge. To address these issues, we utilized two bioinformatics approaches, coefficients of variances and resampling tests, to identify probes showing stable methylation levels from several independent microarray datasets. Mass spectrometry validated the consistently high methylation levels of the five probes (N4BP2, EGFL8, CTRB1, TSPAN3, and ZNF690) in 13 human tissue types from 24 cell lines. Linear associations between detected methylation levels and methyl concentrations of DNA samples were further demonstrated in three genes (N4BP2, EGFL8, and CTRB1). To summarize, we identified five genes which may serve as internal controls for methylation studies by analyzing large-scale microarray data, and three of them can be used as markers for evaluating the efficiency of bisulfite conversion. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896296116&doi=10.1038%2fsrep04351&partnerID=40&md5=e11baf9bcc07c5f1206ef1ae701dda8a https://scholars.lib.ntu.edu.tw/handle/123456789/604928 |
ISSN: | 20452322 24619003 |
DOI: | 10.1038/srep04351 | SDG/關鍵字: | apoptosis regulatory protein; BCL 3 binding protein, human; BCL-3-binding protein, human; biological marker; carrier protein; chymotrypsin; chymotrypsin b; EGFL8 protein, human; endothelial cell growth factor; tetraspanin; article; biology; DNA methylation; DNA microarray; genetics; human; mass spectrometry; metabolism; protein processing; standard; Apoptosis Regulatory Proteins; Biological Markers; Carrier Proteins; Chymotrypsin; Computational Biology; DNA Methylation; Endothelial Growth Factors; Humans; Mass Spectrometry; Oligonucleotide Array Sequence Analysis; Protein Processing, Post-Translational; Reference Standards; Tetraspanins |
顯示於: | 流行病學與預防醫學研究所 |
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