https://scholars.lib.ntu.edu.tw/handle/123456789/605858
標題: | Investigating the reciprocal interrelationships among the ruminal microbiota, metabolome, and mastitis in early lactating holstein dairy cows | 作者: | Chuang S.-T Li K.-Y Tu P.-W Ho S.-T Hsu C.-C Hsieh J.-C MING-JU CHEN CHENG-CHIH HSU |
關鍵字: | Holstein dairy cows;Mastitis;Metabolome;Ruminal microbiota;1 (1h benzo[d]imidazol 2 yl) ethan 1 ol;anacardic acid;formic acid;genomic DNA;imidazole derivative;interleukin 6;pantothenic acid;tumor necrosis factor;unclassified drug;xanthine;xanthurenic acid;animal experiment;animal model;Article;cell count;controlled study;dairy cattle;discriminant analysis;effect size;enzyme linked immunosorbent assay;false discovery rate;female;high performance liquid chromatography;Holstein cattle;mass spectrometry;mastitis;metabolome;metabolomics;microbial interaction;nonhuman;reciprocal interrelationship;rumen fluid;rumen microorganism;Ruminococcus flavefaciens;somatic cell;Treponema;Treponema saccharophilum | 公開日期: | 2021 | 卷: | 11 | 期: | 11 | 來源出版物: | Animals | 摘要: | Mastitis in dairy cow significantly affects animal performance, ultimately reducing profitability. The reciprocal interrelationships among ruminal microbiota, metabolome, and mastitis combining early inflammatory factors (serum proinflammatory cytokines) in lactating dairy cows has not been explored, thus, this study evaluated these reciprocal interrelationships in early lactating Holstein dairy cows to identify potential microbial biomarkers and their relationship with ruminal metabolites. The ruminal fluid was sampled from 8 healthy and 8 mastitis cows for the microbiota and metabolite analyses. The critical ruminal microbial biomarkers and metabolites related to somatic cell counts (SCC) and serum proinflammatory cytokines were identified by the linear discriminant analysis effect size (LEfSe) algorithm and Spearman’s correlation analysis, respectively. The SCC level and proinflammatory cytokines positively correlated with Sharpea and negatively correlated with Ruminococcaceae UCG-014, Ruminococcus flavefaciens, and Treponema saccharophilum. Furthermore, the metabolites xanthurenic acid, and 1-(1H-benzo[d]imidazol-2-yl) ethan-1-ol positively correlated with microbial biomarkers of healthy cows, whereas, xanthine, pantothenic acid, and anacardic acid were negatively correlated with the microbial biomarkers of mastitis cows. In conclusion, Ruminococcus flavefaciens and Treponema saccharophilum are potential strains for improving the health of dairy cows. The current study provides a novel perspective to assist in targeting the ruminal microbiota with preventive/therapeutic strategies against inflammatory diseases in the future. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118200499&doi=10.3390%2fani11113108&partnerID=40&md5=ef15d993af68e5a114de952e05a25a44 https://scholars.lib.ntu.edu.tw/handle/123456789/605858 |
ISSN: | 20762615 | DOI: | 10.3390/ani11113108 |
顯示於: | 動物科學技術學系 |
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