https://scholars.lib.ntu.edu.tw/handle/123456789/605863
標題: | Chinese Herbal Medicine Formula Guizhi Li-Zhong Tang as an Alternative to Antibiotic Feed Additives for Preventing Pneumonia in Piglets through Antioxidant, Anti-Inflammatory, and Antiapoptotic Protection | 作者: | Wang C.-H Wu W.-J Su L.-Y Lu C.-W PEI-HWA WANG Lee M.-C Chuang W.-C Wang S.-E Wu C.-H. |
關鍵字: | antibiotic agent;antiinflammatory agent;antioxidant;caspase 3;Chinese medicinal formula;food additive;Glycyrrhiza glabra root;guizhi li zhong tang;immunoglobulin enhancer binding protein;immunoglobulin G;keishi bukuryo gan;manganese superoxide dismutase;tilmicosin;tumor necrosis factor;unclassified drug;animal experiment;animal model;animal tissue;antiapoptotic activity;antiinflammatory activity;antioxidant activity;apoptosis;Article;Atractylodes macrocephala;Chinese medicine;controlled study;coughing;DPPH radical scavenging assay;eosinophil;ginger;ginseng;immunoglobulin blood level;immunohistochemistry;Kupffer cell;lung parenchyma;male;MTT assay;nonhuman;oxidative stress;piglet;pneumonia;protein expression;survival rate;Western blotting | 公開日期: | 2021 | 卷: | 2021 | 來源出版物: | Evidence-based Complementary and Alternative Medicine | 摘要: | Weanling piglets often develop respiratory diseases such as pneumonia because they encounter substantial environmental stress. This study investigated an alternative herbal feed additive, Guizhi Li-Zhong Tang (GLZ), for preventing pneumonia in weanling piglets. An in vitro experiment demonstrated that GLZ has high antioxidant capacity and low cytotoxicity toward Kupffer cells. In addition, GLZ treatment can alleviate lipopolysaccharide (LPS)-induced damage in Kupffer cells. A total of 94 4-week-old piglets were randomly divided into three groups, which received sham treatment, 0.2% Tilmicosin antibiotic (TAB) treatment, or 0.2% GLZ treatment. Piglets receiving the GLZ treatment had a higher survival rate and higher immunoglobulin G levels but lower allergy-related eosinophil levels and cough incidence than did piglets receiving the sham or 0.2% TAB treatments. Through immunohistochemistry and Western blot analysis, we discovered that piglets receiving the 0.2% GLZ treatment had significantly higher expression of antioxidant-related SOD2 and lower expression of oxidative-stress-related 3-NT (p<0.01), inflammation-related TNF-α (p<0.01) and NF-B (p<0.05), and apoptosis-related caspase-3 (p<0.01) in lung tissue than did piglets receiving the sham or 0.2% TAB treatment. Therefore, GLZ treatment is promising as an alternative to antibiotic medicine for weanling piglets because of its protective antioxidative, anti-inflammatory, and antiapoptotic effects in lung tissue. ? 2021 Che-Hsuan Wang et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115813941&doi=10.1155%2f2021%2f4978783&partnerID=40&md5=c633049351cbf469651344dc104d38b1 https://scholars.lib.ntu.edu.tw/handle/123456789/605863 |
ISSN: | 1741427X | DOI: | 10.1155/2021/4978783 |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。