https://scholars.lib.ntu.edu.tw/handle/123456789/606068
標題: | Cytotoxicity and Apoptosis Induction of 6,7-Dehydroroyleanone from Taiwania cryptomerioides Bark Essential Oil in Hepatocellular Carcinoma Cells | 作者: | Chen G.-R Chang M.-L Chang S.-T Ho Y.-T HUI-TING CHANG SHANG-TZEN CHANG |
關鍵字: | Apoptosis;Cytotoxicity;Hep g2 cell line;Human hepatocellular carcinoma;Taiwania cryptomerioides | 公開日期: | 2022 | 卷: | 14 | 期: | 2 | 來源出版物: | Pharmaceutics | 摘要: | The objective of the present study is to evaluate the cytotoxicity of Taiwania cryptomerioides essential oil and its phytochemical on the Hep G2 cell line (human hepatocellular carcinoma). Bark essential oil has significant cytotoxicity to Hep G2 cells, and S3 fraction is the most active fraction in cytotoxicity to Hep G2 cells among the six fractions. The diterpenoid quinone, 6,7-dehydroroyleanone, was isolated from the active S3 fraction by bioassay-guided isolation. 6,7-Dehydroroyleanone exhibited significant cytotoxicity in Hep G2 cells, and the efficacy of 6,7-dehydroroyleanone was better than the positive control, etoposide. Apoptosis analysis of Hep G2 cells with different treatments was characterized via flow cytometry to confirm the cell death situation. Etoposide and 6,7-dehydroroyleanone could induce the apoptosis in Hep G2 cells using flow cytometric assay. Results revealed 6,7-dehydroroyleanone from T. cryptomerioides bark essential oil can be a potential phytochemical to develop the anticancer chemotherapeutic agent for the treatment of the human hepatocellular carcinoma. ? 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85124104146&doi=10.3390%2fpharmaceutics14020351&partnerID=40&md5=d074b32e6ae8feea6a6e3e1b54a5985b https://scholars.lib.ntu.edu.tw/handle/123456789/606068 |
ISSN: | 19994923 | DOI: | 10.3390/pharmaceutics14020351 |
顯示於: | 森林環境暨資源學系 |
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