https://scholars.lib.ntu.edu.tw/handle/123456789/606177
標題: | IMPDH forms the cytoophidium in zebrafish | 作者: | Keppeke G.D Chang C.-C Antos C.L Peng M Sung L.-Y Andrade L.E.C Liu J.-L. LI-YING SUNG |
關鍵字: | Adult zebrafish;Cytoophidium;IMPDH;Larval zebrafish;6 diazo 5 oxonorleucine;inosinate dehydrogenase;inosine monophosphate dehydrogenase 1a;inosine monophosphate dehydrogenase 1b;inosine monophosphate dehydrogenase 2;isoenzyme;messenger RNA;unclassified drug;guanosine triphosphate;zebrafish protein;adult;animal cell;animal experiment;animal model;animal tissue;Article;cell differentiation;confocal laser scanning microscopy;controlled study;down regulation;embryo;eukaryote;fluorescence microscopy;gene sequence;genetic transfection;HeLa cell line;HEp-2 cell line;human;human cell;image analysis;immunoblotting;immunofluorescence;immunohistochemistry;larva;life cycle stage;nonhuman;oocyte;ovary follicle;ovary follicle cell;point mutation;polymerization;prokaryote;protein expression;retinal inner nuclear layer;retinal inner plexiform layer;retinal outer nuclear layer;retinal outer plexiform layer;reverse transcription polymerase chain reaction;RNA extraction;site directed mutagenesis;upregulation;vertebrate;zebra fish;animal;biosynthesis;cell line;chemistry;cytoplasm;gene expression;genetics;growth, development and aging;metabolism;ultrastructure;Animals;Cell Line;Cytoplasmic Structures;Gene Expression;Guanosine Triphosphate;Humans;IMP Dehydrogenase;Isoenzymes;Point Mutation;Up-Regulation;Zebrafish;Zebrafish Proteins | 公開日期: | 2021 | 卷: | 478 | 起(迄)頁: | 89-101 | 來源出版物: | Developmental Biology | 摘要: | Inosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step in de novo guanine nucleotide biosynthesis. Its activity is negatively regulated by the binding of GTP. IMPDH can form a membraneless subcellular structure termed the cytoophidium in response to certain changes in the metabolic status of the cell. The polymeric form of IMPDH, which is the subunit of the cytoophidium, has been shown to be more resistant to the inhibition by GTP at physiological concentrations, implying a functional correlation between cytoophidium formation and the upregulation of GTP biosynthesis. Herein we demonstrate that zebrafish IMPDH1b and IMPDH2 isoforms can assemble abundant cytoophidium in most of cultured cells under stimuli, while zebrafish IMPDH1a shows distinctive properties of forming the cytoophidium in different cell types. Point mutations that disrupt cytoophidium structure in mammalian models also prevent the aggregation of zebrafish IMPDHs. In addition, we discover the presence of the IMPDH cytoophidium in various tissues of larval and adult fish under normal growth conditions. Our results reveal that polymerization and cytoophidium assembly of IMPDH can be a regulatory machinery conserved among vertebrates, and with specific physiological purposes. ? 2021 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109002632&doi=10.1016%2fj.ydbio.2021.05.017&partnerID=40&md5=eb9a30cb868ac7bb074e35490eacf99a https://scholars.lib.ntu.edu.tw/handle/123456789/606177 |
ISSN: | 00121606 | DOI: | 10.1016/j.ydbio.2021.05.017 |
顯示於: | 生物科技研究所 |
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