https://scholars.lib.ntu.edu.tw/handle/123456789/606178
標題: | CTPS forms the cytoophidium in zebrafish | 作者: | Chang C.-C Keppeke G.D Antos C.L Peng M Andrade L.E.C LI-YING SUNG Liu J.-L. |
關鍵字: | CTPS;Cytoophidium;Zebrafish;6 diazo 5 oxonorleucine;cytidine triphosphate synthase;cytidine triphosphate synthase 1a;cytidine triphosphate synthase 1b;isoprotein;unclassified drug;cytidine triphosphate;ligase;nitric oxide synthase;adult;amino terminal sequence;animal cell;Article;carboxy terminal sequence;cell culture;cell structure;confocal laser scanning microscopy;embryo;gallbladder;gene expression;genetic transfection;gill;heart;HEK293T cell line;human;human cell;human tissue;image analysis;immunofluorescence;intestine;kidney;larva;liver;lymphocyte proliferation;nonhuman;pancreas;point mutation;real time polymerase chain reaction;spleen;tetramerization;transient expression;zebra fish;animal;cell line;cytology;eukaryote;metabolism;prokaryotic cell;Animals;Carbon-Nitrogen Ligases;Cell Line;Cytidine Triphosphate;Eukaryota;Nitric Oxide Synthase;Prokaryotic Cells | 公開日期: | 2021 | 卷: | 405 | 期: | 2 | 來源出版物: | Experimental Cell Research | 摘要: | Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-L-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates. ? 2021 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109199156&doi=10.1016%2fj.yexcr.2021.112684&partnerID=40&md5=c180ecdd972cbf5d9258c921d31d5a92 https://scholars.lib.ntu.edu.tw/handle/123456789/606178 |
ISSN: | 00144827 | DOI: | 10.1016/j.yexcr.2021.112684 |
顯示於: | 生物科技研究所 |
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