https://scholars.lib.ntu.edu.tw/handle/123456789/606179
標題: | CTPS and IMPDH form cytoophidia in developmental thymocytes | 作者: | Peng M Chang C.-C Liu J.-L Sung L.-Y. LI-YING SUNG |
關鍵字: | CTPS;Cytoophidium;Glycolysis;IMPDH;Thymocyte;6 phosphofructokinase;cytidine triphosphate synthase;inosinate dehydrogenase;Myc protein;protein kinase B;cytidine triphosphate;IMPDH1, mouse;ligase;adipose tissue;adult;animal cell;animal experiment;animal tissue;Article;brain;CD4+ T lymphocyte;CD8+ T lymphocyte;cell proliferation;cell structure;colon;controlled study;cytoophidium;enzyme phosphorylation;female;gallbladder;glycolysis;heart;human;intestine;kidney;liver;lung;lymph node;male;mouse;nonhuman;ovary;pancreas;priority journal;prostate;retina;skeletal muscle;skin;spleen;stomach;TCR signaling;testis;thymocyte;thymus;animal;cytology;cytoskeleton;Institute for Cancer Research mouse;metabolism;physiology;signal transduction;Animals;Carbon-Nitrogen Ligases;Cell Proliferation;Cytidine Triphosphate;Cytoskeleton;Female;IMP Dehydrogenase;Male;Mice;Mice, Inbred ICR;Signal Transduction;Thymocytes | 公開日期: | 2021 | 卷: | 405 | 期: | 1 | 來源出版物: | Experimental Cell Research | 摘要: | The cytoophidium, a filamentous structure formed by metabolic enzymes, has emerged as a novel regulatory machinery for certain proteins. The rate-limiting enzymes of de novo CTP and GTP synthesis, cytidine triphosphate synthase (CTPS) and inosine monophosphate dehydrogenase (IMPDH), are the most characterized cytoophidium-forming enzymes in mammalian models. Although the assembly of CTPS cytoophidia has been demonstrated in various organisms including multiple human cancers, a systemic survey for the presence of CTPS cytoophidia in mammalian tissues in normal physiological conditions has not yet been reported. Herein, we examine major organs of adult mouse and observe that CTPS cytoophidia are displayed by a specific thymocyte population ranging between DN3 to early DP stages. Most of these cytoophidium-presenting cells have both CTPS and IMPDH cytoophidia and undergo rapid cell proliferation. In addition, we show that cytoophidium formation is associated with active glycolytic metabolism as the cytoophidium-presenting cells exhibit higher levels of c-Myc, phospho-Akt and PFK. Inhibition of glycolysis with 2DG, however, disrupts most of cytoophidium structures and impairs cell proliferation. Our findings not only indicate that the regulation of CTPS and IMPDH cytoophidia are correlated with the metabolic switch triggered by pre-TCR signaling, but also suggest physiological roles of the cytoophidium in thymocyte development. ? 2021 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106865200&doi=10.1016%2fj.yexcr.2021.112662&partnerID=40&md5=c4e01b056dbef716521d9f21267acf7a https://scholars.lib.ntu.edu.tw/handle/123456789/606179 |
ISSN: | 00144827 | DOI: | 10.1016/j.yexcr.2021.112662 |
顯示於: | 生物科技研究所 |
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