https://scholars.lib.ntu.edu.tw/handle/123456789/606889
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lkhagva A | en_US |
dc.contributor.author | Tai H.-C. | en_US |
dc.contributor.author | HWAN-CHING TAI | zz |
dc.creator | Lkhagva A;Tai H.-C. | - |
dc.date.accessioned | 2022-04-25T06:39:03Z | - |
dc.date.available | 2022-04-25T06:39:03Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 14203049 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120970979&doi=10.3390%2fmolecules26247416&partnerID=40&md5=24c9218831c6db73aab5690df323ad88 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/606889 | - |
dc.description.abstract | Metabolomics profiling using liquid chromatography-mass spectrometry (LC-MS) has become an important tool in biomedical research. However, resolving enantiomers still repre-sents a significant challenge in the metabolomics study of complex samples. Here, we introduced N,N-dimethyl-L-cysteine (dimethylcysteine, DiCys), a chiral thiol, for the o-phthalaldehyde (OPA) derivatization of enantiomeric amine metabolites. We took interest in DiCys because of its potential for multiplex isotope-tagged quantification. Here, we characterized the usefulness of DiCys in reversed-phase LC-MS analyses of chiral metabolites, compared against five commonly used chiral thiols: N-acetyl-L-cysteine (NAC); N-acetyl-D-penicillamine (NAP); isobutyryl-L-cysteine (IBLC); N-(tert-butoxycarbonyl)-L-cysteine methyl ester (NBC); and N-(tert-butylthiocarbamoyl)-L-cysteine ethyl ester (BTCC). DiCys and IBLC showed the best overall performance in terms of chiral separation, fluorescence intensity, and ionization efficiency. For chiral separation of amino acids, DiCys/OPA also outperformed Marfey’s reagents: 1-fluoro-2-4-dinitrophenyl-5-L-valine amide (FDVA) and 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (FDAA). As proof of principle, we compared DiCys and IBLC for detecting chiral metabolites in aqueous extracts of rice. By LC–MS analyses, both methods detected twenty proteinogenic L-amino acids and seven D-amino acids (Ala, Arg, Lys, Phe, Ser, Tyr, and Val), but DiCys showed better analyte separation. We conclude that DiCys/OPA is an excellent amine-derivatization method for enantiomeric metabolite detection in LC-MS analyses. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.relation.ispartof | Molecules | - |
dc.subject | Chiral metabolomics | - |
dc.subject | D-amino acids | - |
dc.subject | Dimethyl labeling | - |
dc.subject | Enantiomer separation | - |
dc.subject | Rice water | - |
dc.title | Dimethylcysteine (Dicys)/o-phthalaldehyde derivatization for chiral metabolite analyses: Cross-comparison of six chiral thiols | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/molecules26247416 | - |
dc.identifier.pmid | 34946495 | - |
dc.identifier.scopus | 2-s2.0-85120970979 | - |
dc.relation.journalvolume | 26 | - |
dc.relation.journalissue | 24 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
顯示於: | 化學系 |
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