https://scholars.lib.ntu.edu.tw/handle/123456789/606943
Title: | Ultrasensitive and Low-Cost Paper-Based Graphene Oxide Nanobiosensor for Monitoring Water-Borne Bacterial Contamination | Authors: | Karuppiah S Mishra N.C Tsai W.-C WEI-SSU LIAO Chou C.-F. |
Keywords: | E. coli;graphene;impedimetric sensing;paper-based biosensors;screen-printed electrode;water-borne pathogens;Biosensors;Charge transfer;Cost effectiveness;Costs;Effluents;Electrochemical impedance spectroscopy;Electron transitions;Electron transport properties;Escherichia coli;Graphene;Pollution detection;Sewage sludge;Bacterial contamination;Biorecognition elements;Charge transfer resistance;Electroactive surface areas;Electrochemical characterizations;Fast electron transfer;Label-free biosensor;Water-borne pathogens;Water pollution;graphene oxide;graphite;water;Graphite;Water | Issue Date: | 2021 | Journal Volume: | 6 | Journal Issue: | 9 | Start page/Pages: | 3214-3223 | Source: | ACS Sensors | Abstract: | Water-borne pathogens are mostly generated due to poor sanitation, industrial effluents, and sewage sludge, leading to a significant increase in mortality rate. To prevent this, we need a simple, user-friendly, and rapid on-site detection tool of pathogens, i.e., a biosensor. As contaminated water mainly contains (80%) coliform bacteria, of whichEscherichia coliis the major species, we have developed a screen-printed paper-based, label-free biosensor for the detection ofE. coliin water. A nanoarchitectured graphene oxide (GO), as a fast electron-transfer flatland, was deposited on the screen-printed graphene (G) on a hydrophobic paper, followed by the immobilization of lectin Concanavalin A (ConA) as a biorecognition element for a GGO_ConA-biosensing electrode. The electrochemical characterization of GGO_ConA shows fast electron transfer with a calculated electroactive surface area of 0.16 cm2. The biosensor performance was tested in the sludge water and beach water (real sample) as an analyte using the electrochemical impedance spectroscopy (EIS) technique. The charge-transfer resistance (Rct) of GGO_ConA increases linearly with the bacterial concentration in the range of 10-108CFU mL-1with an estimated limit of detection (LOD) of 10 CFU mL-1, which indicates the ultrasensitivity of our biosensor, with 100 times more sensitivity than previous studies. Our reported biosensor, being cost-effective, eco-friendly, and ultrasensitive, may serve greatly as a portable monitoring kit for checking water-borne bacterial contamination. ? 2021 American Chemical Society |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114676745&doi=10.1021%2facssensors.1c00851&partnerID=40&md5=90ded5a1c7fdc58ea88cb48165b6d061 https://scholars.lib.ntu.edu.tw/handle/123456789/606943 |
ISSN: | 23793694 | DOI: | 10.1021/acssensors.1c00851 |
Appears in Collections: | 化學系 |
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