|Title:||Amperometric detection of morphine at a Prussian blue-modified indium tin oxide electrode||Authors:||Hoa, Kuo-Chuan
|Keywords:||Amperometric detection;Electrochemical sensor;Artificial peroxidase;Morphine;Prussian blue||Issue Date:||2004||Publisher:||Taipei:National Taiwan University Dept Chem Engn||Journal Volume:||20||Journal Issue:||1||Start page/Pages:||3-8||Source:||Biosensors & Bioelectronics 20(2004), 3–8||Abstract:||
In this work, the electrocatalytic oxidation of morphine (MO) at an optically transparent indium tin oxide (ITO) electrode modified by an
electrodeposited Prussian blue (PB) thin film is first demonstrated, and the amperometric detection ofMOwas then investigated. Experimental
results showed that the thin film on the ITO surface, confined to the PB/Berlin green (BG) redox pair, can serve as an excellent mediator
which facilitates electron transfer and considerably lowers the overpotential required, as compared to a bare ITO electrode. Thus, PB can be
regarded as a promising artificial peroxidase for MO. The rate of such an electrocatalytic reaction is pH dependent with the highest value at
pH 5. By potential-step excitation from 0.55 to 0.70V, a linear calibration curve, displaying the relationship between steady-state currents
and MO concentrations (ranging from 0.09 to 1.0 mM), was obtained. The detection sensitivity is about 16.8A/cm2 mM. Most importantly,
the method described herein can readily discriminate MO analogs lacking the phenolic –OH group, such as codeine, and can thus benefit the
specific recognition of MO.
|Appears in Collections:||化學工程學系|
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