https://scholars.lib.ntu.edu.tw/handle/123456789/61258
標題: | Amperometric detection of morphine at a Prussian blue-modified indium tin oxide electrode | 作者: | KUO-CHUAN HO Chen, Chia-Yi Hsu, Huan-Cheng Chen, Lin-Chi Shiesh, Shu-Chu Lin, Xi-Zhang |
關鍵字: | Amperometric detection;Electrochemical sensor;Artificial peroxidase;Morphine;Prussian blue | 公開日期: | 2004 | 出版社: | Taipei:National Taiwan University Dept Chem Engn | 卷: | 20 | 期: | 1 | 起(迄)頁: | 3-8 | 來源出版物: | Biosensors & Bioelectronics | 摘要: | In this work, the electrocatalytic oxidation of morphine (MO) at an optically transparent indium tin oxide (ITO) electrode modified by an electrodeposited Prussian blue (PB) thin film is first demonstrated, and the amperometric detection ofMOwas then investigated. Experimental results showed that the thin film on the ITO surface, confined to the PB/Berlin green (BG) redox pair, can serve as an excellent mediator which facilitates electron transfer and considerably lowers the verpotential required, as compared to a bare ITO electrode. Thus, PB can be regarded as a promising artificial peroxidase for MO. The rate of such an electrocatalytic reaction is pH dependent with the highest value at pH 5. By potential-step excitation from 0.55 to 0.70V, a linear calibration curve, displaying the relationship between steady-state currents and MO concentrations (ranging from 0.09 to 1.0 mM), was obtained. The detection sensitivity is about 16.8A/cm2 mM. Most importantly, the method described herein can readily discriminate MO analogs lacking the phenolic –OH group, such as codeine, and can thus benefit the specific recognition of MO. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/2006111501232967 http://ntur.lib.ntu.edu.tw/bitstream/246246/2006111501232967/1/4729.pdf |
其他識別: | 246246/2006111501232967 | DOI: | 10.1016/j.bios.2003.11.027 |
顯示於: | 化學工程學系 |
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