https://scholars.lib.ntu.edu.tw/handle/123456789/613292
標題: | Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred bioreactors with a stainless steel net | 作者: | KUNG-TA LEE Suzuki, T. Yamakawa, T. Kodama, T. Igarashi, Y. Shimomura, K. |
關鍵字: | Atropa belladonna | Modified stirred bioreactors | Scale-up | Transformed root cultures | Tropane alkaloids | 公開日期: | 1-三月-1999 | 卷: | 18 | 期: | 7-8 | 起(迄)頁: | 567-571 | 來源出版物: | Plant Cell Reports | 摘要: | A scale up of transformed root cultures of Atropa belladonna from a 300-ml flask to a 30-1 tank was accomplished without any reduction in alkaloid productivity. Cutting treatment of seed cultures showed no distinct effect on root growth, morphology, and alkaloid content in conical flasks during 1 month of culture. Randomly cut roots thus grown were further cultivated in 3-1 and 30-1 modified stirred bioreactors for a scale-up culture. After 1 month of culture, 1490 mg of tropane alkaloids was produced by a 30-1 culture of A. belladonna transformed roots. These roots contained the same level of atropine (5.4 mg/g dw) as the roots of this plant grown in the field for 12 months and still contained a considerable amount of other alkaloids including 1.6 mg/g dw of 6-β-hydroxyhyoscyamine, 0.9 mg/g dw of scopolamine, and 2.0 mg/g dw of littorine. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/613292 | ISSN: | 07217714 | DOI: | 10.1007/s002990050623 |
顯示於: | 生化科技學系 |
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