https://scholars.lib.ntu.edu.tw/handle/123456789/616148
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Li, Jing | en_US |
dc.contributor.author | WEI-TIEN CHANG | en_US |
dc.contributor.author | Qin, Gina | en_US |
dc.contributor.author | Wojcik, Kimberly R | en_US |
dc.contributor.author | Li, Chang-Qing | en_US |
dc.contributor.author | Hsu, Chin-Wang | en_US |
dc.contributor.author | Han, Mei | en_US |
dc.contributor.author | Zhu, Xiangdong | en_US |
dc.contributor.author | Vanden Hoek, Terry L | en_US |
dc.contributor.author | Shao, Zuo-Hui | en_US |
dc.date.accessioned | 2022-08-03T07:14:15Z | - |
dc.date.available | 2022-08-03T07:14:15Z | - |
dc.date.issued | 2022-06-23 | - |
dc.identifier.issn | 0192415X | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/616148 | - |
dc.description.abstract | Preconditioning has a powerful protective potential against myocardial ischemia-reperfusion injury (I/R). Our prior work demonstrated that baicalein, a flavonoid derived from the root of Scatellaria baicalensis Georgi (also known as Huangqin), confers this preconditioning protection. This study further explored the mechanisms of baicalein preconditioning (BC-PC) in mouse cardiomyocytes. Cells were treated with baicalein (10 [Formula: see text] M) for a brief period of time (10 min) prior to simulated ischemia 90 min/reperfusion for 180 min. Baicalein triggered an induction of a small amount of mitochondrial reactive oxygen species (ROS) prior to the initiation of ischemia, assessed by 6-carboxy-2', 7'-dichlorodihydrofluorescein diacetate (6-carboxy-H2DCFDA). It also significantly increased cell viability measured by propidium iodide (PI) and lactate dehydrogenase and preserved mitochondrial membrane potential assessed by TMRM fluorescence intensity. Myxothiazol, a mitochondrial electron transport chain complex III inhibitor, partially blocked ROS generation induced by BC-PC and reduced cell viability. BC-PC increased phosphorylation of Akt (Thr308 and Ser473) and eNOS Ser1177, and nitric oxide (NO) production measured using 4,5-diaminofluorescein diacetate (DAF-2 DA, 1 [Formula: see text] M). Akt inhibitor API-2 abolished Akt phosphorylation and reduced DAF-2 production and cell viability. In addition, BC-PC decreased phosphorylation of pyruvate dehydrogenase (PDH) reflecting upregulated PDH activity, and increased ATP production at 30 min during reperfusion. Taken together, baicalein preconditioning-induced cardioprotection involves pro-oxidant generation, activates survival signaling Akt/eNOS/NO, and improves metabolic recovery after I/R injury. Our work provides new perspectives on the effect of baicalein on cardiac preconditioning against I/R injury. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | The American journal of Chinese medicine | en_US |
dc.subject | Akt; Baicalein Preconditioning; Ischemia/Reperfusion; Mitochondrial Complex III; Pro-Oxidant; Pyruvate Dehydrogenase; ROS | en_US |
dc.title | Baicalein Preconditioning Cardioprotection Involves Pro-Oxidant Signaling and Activation of Pyruvate Dehydrogenase | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1142/S0192415X22500513 | - |
dc.identifier.pmid | 35748215 | - |
dc.identifier.scopus | 2-s2.0-85132975857 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85132975857 | - |
dc.relation.pageend | 13 | en_US |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Emergency Medicine | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-1880-7932 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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