https://scholars.lib.ntu.edu.tw/handle/123456789/616179
標題: | Chitosan Inhibits the Rehabilitation of Damaged Microbes Induced by Photodynamic Inactivation | 作者: | Lin, Ching-Hsuan Chien, Hsiung-Fei Lin, Ming-Hsuan Chen, Chueh-Pin Shen, Mandy C. T. Chen C. H. Lin |
關鍵字: | cell wall; microbial killing; photodynamic treatment | 公開日期: | 1-九月-2018 | 出版社: | MDPI | 卷: | 19 | 期: | 9 | 來源出版物: | International journal of molecular sciences | 摘要: | Previously, we showed that chitosan could augment the biocidal efficacy mediated by photodynamic treatment against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. In this study, we showed that the antimicrobial action of chitosan in augmenting photodynamic inactivation (PDI) is related to the increase in cell surface destruction. The microbial cell surfaces exhibit severe irregular shapes after PDI in the presence of chitosan as demonstrated by transmitted electron microscopy. Furthermore, increases in the concentration or incubation time of chitosan significantly reduced the amounts of photosensitizer toluidine blue O required, indicating that chitosan could be an augmenting agent used in conjunction with PDI against S. aureus, P. aeruginosa, and C. albicans. A prolonged lag phase was found in microbial cells that survived to PDI, in which chitosan acted to completely eradicate the cells. Once the exponential log stage and cell rebuild began, their cellular functions from PDI-induced damage returned and the increased cytotoxic effect of chitosan disappeared. Together, our results suggest that chitosan can prevent the rehabilitation of PDI-surviving microbial cells, leading to increased biocidal efficacy. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/616179 | ISSN: | 1422-0067 | DOI: | 10.3390/ijms19092598 |
顯示於: | 生化科技學系 |
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Chitosan inhibits the rehabilitation of damaged microbes induced by photodynamic inactivation.pdf | 3.6 MB | Adobe PDF | 檢視/開啟 |
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