https://scholars.lib.ntu.edu.tw/handle/123456789/620738
標題: | Investigation of the mechanism of transdermal penetration enhancer: A comparison of multiphoton microscopy and electron microscopy | 作者: | SUNG-JAN LIN Lee, J.-N. Lin, C.-Y. CHIH-CHIEH CHAN Lin, M.-G. Wang, C.-C. Tan, H.-Y. Tsai, T.-H. Jee, S.-H. CHEN-YUAN DONG |
公開日期: | 2008 | 卷: | 6842 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | The aim of this study is to characterize the ability of multiphoton microscopy in monitoring the transdermal penetration enhancing effect of a depilatory agent and the associated structural alterations of stratum corneum. The result is compared with the electron microscopic findings. Our results show that the penetration of both hydrophilic and hydrophobic agents can be enhanced. The morphology of corneocytes becomes a homogenized pattern with focal detachment of surface corneocytes. In combination with Nile red staining, multiphoton imaging also shows that the regular motar-like distribution of lipid matrix was disrupted into a homogenized pattern of lipid distribution. These results are well correlated with the findings of ultrastructural analysis by electron micrographs showing disintegration of the protein envelope of coenocytes, disruption of intracellular keratin and loss of the regular lamellar packing of intercellular lipids. We conclude that, in addition to quantifying the permeation profiles of model drugs, multiphoton microscopy is able to detect the penetration enhancer-induced structural alterations of stratum corneum. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-42149114691&doi=10.1117%2f12.761429&partnerID=40&md5=9c4caf6e7f53c11ba47d8ec61fc9bd53 https://scholars.lib.ntu.edu.tw/handle/123456789/620738 |
ISSN: | 1605-7422 | DOI: | 10.1117/12.761429 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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