https://scholars.lib.ntu.edu.tw/handle/123456789/621066
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHIA-TI TSAI | en_US |
dc.contributor.author | FU-TIEN CHIANG | en_US |
dc.contributor.author | WEN-PIN CHEN | en_US |
dc.contributor.author | HWANG, JUEY-JEN | en_US |
dc.contributor.author | Tseng, Chuen-Den | en_US |
dc.contributor.author | CHO-KAI WU | en_US |
dc.contributor.author | CHIH-CHIEH YU | en_US |
dc.contributor.author | YI-CHIH WANG | en_US |
dc.contributor.author | LING-PING LAI | en_US |
dc.contributor.author | JIUNN-LEE LIN | en_US |
dc.date.accessioned | 2022-09-19T07:32:22Z | - |
dc.date.available | 2022-09-19T07:32:22Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.issn | 0143-4160 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/621066 | - |
dc.description.abstract | Angiotensin II (AngII) has been implicated in the mechanism of atrial fibrillation (AF). There may be calcium-dependent pro-fibrillatory effect of AngII on atrial myocytes. We used cultured confluent HL-1 atrial myocyte monolayer with spontaneously propagated depolarization to study direct pro-fibrillatory effect of AngII and its molecular mechanism. AngII stimulation induced fibrillatory-like complex electrogram and calcium wave propagation. AngII shortened action potential duration and augmented calcium transient, thus increasing electrochemical gradient of forward-mode sodium-calcium exchanger (NCX) current and induced frequent irregular afterdepolarizations. AngII increased expression of sodium-calcium exchanger (NCX), further increasing calcium-membrane voltage coupling gain. The fibrillatory effect of AngII was attenuated by NCX blocker SEA0400 and NCX siRNA knockdown. AngII increased expression of L-type calcium channel and augmented calcium transient through PKC and CREB. The fibrillatory effect of AngII was also attenuated by PKC inhibitor chelerythrine and dominant negative form of CREB. In conclusions, AngII itself may electrically contribute to the mechanism of AF through increasing NCX expression and augmenting calcium transient, which is PKC and CREB dependent. Specific genetic knockdown of NCX attenuated calcium mediated afterdepolarization and complex electrogram. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.relation.ispartof | Cell calcium | en_US |
dc.subject | Angiotensin II; Atrial fibrillation; Cardiomyocyte monolayer; Sodium-calcium exchanger; Signal transduction; CONVERTING ENZYME-INHIBITION; CARDIAC FIBROBLASTS; HEART-FAILURE; REPOLARIZATION ALTERNANS; MYOCARDIAL-INFARCTION; TRIGGERED ACTIVITY; GENE-EXPRESSION; SUDDEN-DEATH; SINUS RHYTHM; CELL-LINE | en_US |
dc.title | Angiotensin II induces complex fractionated electrogram in a cultured atrial myocyte monolayer mediated by calcium and sodium-calcium exchanger | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.ceca.2010.10.005 | - |
dc.identifier.pmid | 21168206 | - |
dc.identifier.scopus | 2-s2.0-79951550860 | - |
dc.identifier.isi | WOS:000288735400001 | - |
dc.identifier.url | https://scholars.lib.ntu.edu.tw/handle/123456789/556558 | - |
dc.relation.pages | 1 | en_US |
dc.relation.journalvolume | 49 | en_US |
dc.relation.journalissue | 1 | en_US |
dc.relation.pageend | 11 | en_US |
item.languageiso639-1 | en | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Pharmacology | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-4853-8665 | - |
crisitem.author.orcid | 0000-0003-4936-8968 | - |
crisitem.author.orcid | 0000-0002-0143-5642 | - |
crisitem.author.orcid | 0000-0001-6437-0455 | - |
crisitem.author.orcid | 0000-0002-3867-150X | - |
crisitem.author.orcid | 0000-0002-2136-2444 | - |
crisitem.author.orcid | 0000-0003-2533-865X | - |
crisitem.author.orcid | 0000-0002-9651-7420 | - |
crisitem.author.orcid | 0000-0003-2148-3956 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
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