https://scholars.lib.ntu.edu.tw/handle/123456789/624348
標題: | The phenomenon of staphylococcal interbacterial aggregate and net structures (SIAN) in community-associated methicillin-resistant Staphylococcus aureus CA-MRSA/J | 作者: | Wan, Tsai-Wen Mori, Hiromitsu Hung, Wei-Chun LEE-JENE TENG Yamamoto, Tatsuo |
關鍵字: | CA-MRSA/J; Community-associated methicillin-resistant Staphylococcus aureus; Long/jerky spike; Staphylococcal interbacterial aggregate/net | 公開日期: | 十一月-2022 | 出版社: | ELSEVIER | 卷: | 28 | 期: | 11 | 起(迄)頁: | 1584 | 來源出版物: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy | 摘要: | Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) usually causes skin and soft tissue infections (SSTIs), but occasionally causes invasive infections with a broad range of manifestations. Virulence factors and pathogenesis of CA-MRSA have been investigated in details with genotype ST8/SCCmecIVa (USA300), which first emerged in the United States. However, CA-MRSA evolves rapidly, with different clones dominating in different world regions; their pathogenesis remains unclear. CA-MRSA with genotype ST8/SCCmecIVl (CA-MRSA/J) emerged in 2003 in Japan, spreading widely with a fatal case. We have studied the genetic characteristics of CA-MRSA/J, and during the course of this study, we found that CA-MRSA/J has bacteriophage-like spikes with or without a hexagonal cap (spikes X and Xc). Here, we report that CA-MRSA/J strain NN55 has non-phage-like, one-μm-long/jerky spikes with or without a hexagonal cap (LSX/LSXc), and also that LSX/LSXc forms (staphylococcal) interbacterial aggregate/net structures (SIAN). Regarding the phenomenon of SIAN, NN55 first formed single short spike X, followed by multiple molecules of long and jerky LSX/LSXc, leading to the interbacterial construction of SIAN, in colonies with high cell densities. The LSX/LSXc and SIAN structures have not been reported in S. aureus. NN55 was invasive in a HEp-2 cell assay, exhibiting SIAN. The novel SIAN structures may be foci-skeletons or toxic aggregates in NN55's invasive infections. The phenomenon of SIAN suggests novel staphylococcal cell-surface dynamism, providing a new structure-and-function relationship model and advancing the understanding of CA-MRSA pathogenesis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/624348 | ISSN: | 1341-321X | DOI: | 10.1016/j.jiac.2022.07.024 |
顯示於: | 醫學檢驗暨生物技術學系 |
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