https://scholars.lib.ntu.edu.tw/handle/123456789/625712
標題: | Anti-adipogenic activities of pheophorbide a and pyropheophorbide a isolated from wild bitter gourd (Momordica charantia L. var. abbreviata Seringe) in vitro | 作者: | Liu Y.-H Lu Y.-L Chang Y.-C Chyuan J.-H TZONG-HUEI LEE Hou W.-C. |
關鍵字: | adipocytes; anti-adipogenic activity; differentiation; lactate dehydrogenase; wild bitter gourd (WBG) | 公開日期: | 2022 | 來源出版物: | Journal of the Science of Food and Agriculture | 摘要: | BACKGROUND: The wild bitter gourd (WBG) is a commonly consumed vegetable in Asia that has antioxidant and hypoglycemic properties. The present study aimed to investigate the anti-adipogenic activities of isolated compounds from WBG on 8-day differentiated cultures of 3 T3-L1 adipocytes that were then stained with Oil Red O (ORO) or diamidino-2-phenylindole (DAPI). RESULTS: ORO stains of the methanol extracts of de-seeded HM86 cultivar of WBG (WBG-M) and the ethyl acetate fractions (WBG-M-EA) showed anti-adipogenic activities against differentiated adipocytes. Two chlorophyll-degraded compounds, pheophorbide a (1) and pyropheophorbide a (2), were isolated from WBG-M-EA. Treatments with 1 (5, 10, and 20 μmol L−1) and 2 (2.5, 5, and 10 μmol L−1) showed dose-dependent reductions in lipid accumulations and reduced nuclear DAPI stains in differentiated 3 T3-L1 adipocytes. The concentrations for 50% inhibition against lipid accumulations of 1 and 2, respectively, were 16.05 and 7.04 μmol L−1. Treatments with 1 and 2 showed enhanced lactate dehydrogenase release in the first 4-day cell mitotic clonal expansions during the differentiating cultural processes, although the effect was less on the non-differentiating cultural processes. Thus, 1 and 2 were more toxic to differentiating adipocytes than to non-differentiated pre-adipocytes, which partly resulted in anti-adipogenic activities with lowered lipid accumulations. CONCLUSION: Both 1 and 2 showed anti-adipogenic activities in cell models. These chlorophyll-degraded compounds commonly exist in several vegetables during storage or edible seaweeds, which will provide resources for further investigations aiming to test anti-obesity in animal studies. © 2022 Society of Chemical Industry. © 2022 Society of Chemical Industry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132076009&doi=10.1002%2fjsfa.12044&partnerID=40&md5=8f68603c32d632722f32034339ac96e0 https://scholars.lib.ntu.edu.tw/handle/123456789/625712 |
ISSN: | 00225142 | DOI: | 10.1002/jsfa.12044 |
顯示於: | 漁業科學研究所 |
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