https://scholars.lib.ntu.edu.tw/handle/123456789/626154
Title: | SSR individual identification system construction and population genetics analysis for Chamaecyparis formosensis | Authors: | Huang C.-J FANG-HUA CHU Huang Y.-S Tu Y.-C Hung Y.-M Tseng Y.-H Pu C.-E Hsu C.T Chao C.-H Chou Y.-S Liu S.-C You Y.T Hsu S.-Y Hsieh H.-C Wang C.-T Chen C.-T. |
Issue Date: | 2022 | Journal Volume: | 12 | Journal Issue: | 1 | Source: | Scientific Reports | Abstract: | Chamaecyparis formosensis is an endemic species of Taiwan, threatened from intensive use and illegal felling. An individual identification system for C. formosensis is required to provide scientific evidence for court use and deter illegal felling. In this study, 36 polymorphic simple sequence repeat markers were developed. By applying up to 28 non-linked of the developed markers, it is calculated that the cumulative random probability of identity (CPI) is as low as 1.652 × 10–12, and the identifiable population size is up to 60 million, which is greater than the known C. formosensis population size in Taiwan. Biogeographical analysis data show that C. formosensis from four geographic areas belong to the same genetic population, which can be further divided into three clusters: SY (Eastern Taiwan), HV and GW (Northwestern Taiwan), and MM (Southwestern Taiwan). The developed system was applied to assess the provenance of samples with 88.44% accuracy rate and therefore can serve as a prescreening tool to reduce the range required for comparison. The system developed in this study is a potential crime-fighting tool against illegal felling. © 2022, The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85126078382&doi=10.1038%2fs41598-022-07870-5&partnerID=40&md5=f424de4ef21ed4b1a57d70399f9d438f https://scholars.lib.ntu.edu.tw/handle/123456789/626154 |
ISSN: | 20452322 | DOI: | 10.1038/s41598-022-07870-5 | SDG/Keyword: | article; Chamaecyparis; crime; genetic marker; illegal timber; nonhuman; population genetics; population size; probability; simple sequence repeat; Taiwan; genetics; population genetics; microsatellite DNA; Chamaecyparis; Genetics, Population; Microsatellite Repeats; Taiwan |
Appears in Collections: | 森林環境暨資源學系 |
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