https://scholars.lib.ntu.edu.tw/handle/123456789/626258
標題: | Effective assay of bacterial transglycosylation by molecular turn-on sensing and a second-order scattering effect | 作者: | Hsu T.-W JIM-MIN FANG |
公開日期: | 2021 | 卷: | 146 | 期: | 19 | 起(迄)頁: | 5843-5847 | 來源出版物: | Analyst | 摘要: | Instead of using the lipid II substrate that requires prior labelling with a radioactive isotope or fluorophore to probe the formation of peptidoglycan in bacterial transglycosylation, the released undecaprenyl pyrophosphate (UPP) product is quantitatively measured either using a terpyridine-zinc fluorescence turn-on sensor or simply by the second-order scattering effect of the in situ formed UPP-calcium complex. Both the assay methods are utilized to identify moenomycin A as a potent transglycosylase inhibitor with a consistent IC50 value. This journal is © The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116336658&doi=10.1039%2fd1an00941a&partnerID=40&md5=59e49a56f5726ecc5a27f3cbc2ae2b2a https://scholars.lib.ntu.edu.tw/handle/123456789/626258 |
ISSN: | 00032654 | DOI: | 10.1039/d1an00941a | SDG/關鍵字: | Calcium compounds; Fluorophores; Assay methods; Calcium complexes; IC50 value; Labelings; Peptidoglycans; Scattering effects; Second order scattering; Terpyridines; trans-Glycosylation; Turn-on sensors; Glycosylation; peptidoglycan; bacterium; cell wall; Bacteria; Cell Wall; Peptidoglycan |
顯示於: | 化學系 |
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